ArticleJournal of immunological methods2008
Design and testing of PCR primers for the construction of scFv libraries representing the immunoglobulin repertoire of rats.
Article in Journal of immunological methods, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 28 citations in OpenAlex.
- Development of novel canine phage display-derived neutralizing monoclonal antibody fragments against rabies virus from immunized dogs.Scientific reports · 2024Article
- A primer set for the rapid isolation of scFv fragments against cell surface antigens from immunised rats.Scientific reports · 2020Article
- Screening Antibody Libraries with Colony Assay Using scFv-Alkaline Phosphatase Fusion Proteins.Molecules (Basel, Switzerland) · 2020Article
- Sites of vulnerability on ricin B chain revealed through epitope mapping of toxin-neutralizing monoclonal antibodies.PloS one · 2020Article
- Schistosoma mansoni Infection of Mice, Rats and Humans Elicits a Strong Antibody Response to a Limited Number of Reduction-Sensitive Epitopes on Five Major Tegumental Membrane Proteins.PLoS neglected tropical diseases · 2017Article
- Generation and Characterization of a New Monoclonal Antibody Against CXCL4.Monoclonal antibodies in immunodiagnosis and immunotherapy · 2015Article
- Camelid single domain antibodies (VHHs) as neuronal cell intrabody binding agents and inhibitors of Clostridium botulinum neurotoxin (BoNT) proteases.Toxicon : official journal of the International Society on Toxinology · 2010Article
- Schistosoma mansoni host-exposed surface antigens characterized by sera and recombinant antibodies from schistosomiasis-resistant rats.International journal for parasitology · 2010Article
- Efficient serum clearance of botulinum neurotoxin achieved using a pool of small antitoxin binding agents.Infection and immunity · 2010Article
- Identification and characterization of a novel cell-penetrating peptide.Biochemical and biophysical research communications · 2009Article
- Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Rats are widely used as a model in the study of many important diseases, yet their utility in research is limited by the lack of robust technology for the production of rat recombinant antibodies. Here we have identified and compiled putative rat immunoglobulin sequences by bioinformatic analysis of recently available genomic and EST databases, and used this information to design PCR primers to amplify rat V(H) and V(L) domain coding DNA representing the expressed repertoire of the animal. These primer sets were successfully tested and used to produce a scFv phage display library from rats that had been infected by the blood fluke, Schistosoma mansoni. The library was selected for scFvs that bind to extracellular loop epitopes on either of two known immunogenic S. mansoni membrane proteins, Sm23 and SmTsp2, both of the tetraspanin protein family. Two unique scFvs were identified that bind to each tetraspanin and shown to have excellent specificity for the target, including recognition of the native tetraspanins produced by the fluke. The primers and methods developed for rat scFvs should be of use to others seeking to characterize the antibody repertoire and/or prepare recombinant antibodies from this model.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.