ArticleClinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association2008
Alterations in mucosal immunity identified in the colon of patients with irritable bowel syndrome.
Article in Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers, 2 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
57 citing papers in PubMed, 2 syntheses or guidelines pooled it, 124 citations in OpenAlex.
- Evaluation of gene expression classification studies: factors associated with classification performance.PloS one · 2014Pooled it
- Systematic review of modulators of benzodiazepine receptors in irritable bowel syndrome: is there hope?World journal of gastroenterology · 2011Pooled it
- Review
- What Has Longitudinal 'Omics' Studies Taught Us about Irritable Bowel Syndrome? A Systematic Review.Metabolites · 2023Review
- A review of neuroendocrine immune system abnormalities in IBS based on the brain-gut axis and research progress of acupuncture intervention.Frontiers in neuroscience · 2023Review
- Differential mRNA expression in ileal and colonic biopsies in irritable bowel syndrome with diarrhea or constipation.American journal of physiology. Gastrointestinal and liver physiology · 2022Article
- Article
- Targeted therapy of irritable bowel syndrome with anti-inflammatory cytokines.Clinical journal of gastroenterology · 2022Review
- Multiple rather than specific autoantibodies were identified in irritable bowel syndrome with HuProt™ proteome microarray.Frontiers in physiology · 2022Article
- Expression of TRP Channels in Colonic Mucosa of IBS-D Patients and Its Correlation with the Severity of the Disease.Gastroenterology research and practice · 2022Article
- Article
- Observational
- Gastric Mucosal Immune Profiling and Dysregulation in Idiopathic Gastroparesis.Clinical and translational gastroenterology · 2021Observational
- Intestinal IL-17R Signaling Controls Secretory IgA and Oxidase Balance inJournal of immunology (Baltimore, Md. : 1950) · 2021Article
- Symbioflor2Microorganisms · 2020Article
- Mucosal Microbiome Profiles Polygenic Irritable Bowel Syndrome in Mestizo Individuals.Frontiers in cellular and infection microbiology · 2020Article
- The Gut Microbiome in Adult and Pediatric Functional Gastrointestinal Disorders.Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association · 2019Review
- NADPH oxidases and ROS signaling in the gastrointestinal tract.Mucosal immunology · 2018Review
- Sigmoid colon mucosal gene expression supports alterations of neuronal signaling in irritable bowel syndrome with constipation.American journal of physiology. Gastrointestinal and liver physiology · 2018Article
- Does the number of mucosal immune cells differ in irritable bowel syndrome and its subtypes?The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
16 authors at 2 institutions in 2 countries.
Funding
Abstract
BACKGROUND &
aimsIrritable bowel syndrome (IBS) has been associated with mucosal dysfunction, mild inflammation, and altered colonic bacteria. We used microarray expression profiling of sigmoid colon mucosa to assess whether there are stably expressed sets of genes that suggest there are objective molecular biomarkers associated with IBS.
methodsGene expression profiling was performed using Human Genome U133 Plus 2.0 (Affymetrix) GeneChips with RNA from sigmoid colon mucosal biopsy specimens from 36 IBS patients and 25 healthy control subjects. Real-time quantitative polymerase chain reaction was used to confirm the data in 12 genes of interest. Statistical methods for microarray data were applied to search for differentially expressed genes, and to assess the stability of molecular signatures in IBS patients.
resultsMucosal gene expression profiles were consistent across different sites within the sigmoid colon and were stable on repeat biopsy over approximately 3 months. Differentially expressed genes suggest functional alterations of several components of the host mucosal immune response to microbial pathogens. The most strikingly increased expression involved a yet uncharacterized gene, DKFZP564O0823. Identified specific genes suggest the hypothesis that molecular signatures may enable distinction of a subset of IBS patients from healthy controls. By using 75% of the biopsy specimens as a validation set to develop a gene profile, the test set (25%) was predicted correctly with approximately 70% accuracy.
conclusionsMucosal gene expression analysis shows there are relatively stable alterations in colonic mucosal immunity in IBS. These molecular alterations provide the basis to test the hypothesis that objective biomarkers may be identified in IBS and enhance understanding of the disease.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.