ArticleHistochemistry and cell biology2008
Dual-immunohistochemistry provides little evidence for epithelial-mesenchymal transition in pulmonary fibrosis.
Article in Histochemistry and cell biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 68 citations in OpenAlex.
- Wilms Tumor 1-Driven Fibroblast Activation and Subpleural Thickening in Idiopathic Pulmonary Fibrosis.International journal of molecular sciences · 2023Review
- Minute Cellular Nodules as Early Lesions in Rats with Silica Exposure via Inhalation.Veterinary sciences · 2022Article
- Targeting Alveolar Repair in Idiopathic Pulmonary Fibrosis.American journal of respiratory cell and molecular biology · 2021Review
- Effects ofMicroorganisms · 2021Article
- Fluorofenidone attenuates paraquat‑induced pulmonary fibrosis by regulating the PI3K/Akt/mTOR signaling pathway and autophagy.Molecular medicine reports · 2021Article
- Mefunidone Ameliorates Bleomycin-Induced Pulmonary Fibrosis in Mice.Frontiers in pharmacology · 2021Article
- Fibroblast Growth Factor 2 Augments Transforming Growth Factor Beta 1 Induced Epithelial-mesenchymal Transition in Lung Cell Culture Model.Iranian journal of allergy, asthma, and immunology · 2020Article
- Scarred Lung. An Update on Radiation-Induced Pulmonary Fibrosis.Frontiers in medicine · 2020Review
- Arctigenin Suppressed Epithelial-Mesenchymal Transition Through Wnt3a/β-Catenin Pathway in PQ-Induced Pulmonary Fibrosis.Frontiers in pharmacology · 2020Article
- Tannic acid prevents macrophage-induced pro-fibrotic response in lung epithelial cells via suppressing TLR4-mediated macrophage polarization.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2019Article
- Pathological Study on Epithelial-Mesenchymal Transition in Silicotic Lung Lesions in Rat.Veterinary sciences · 2019Article
- A standardized herbal extract PM014 ameliorates pulmonary fibrosis by suppressing the TGF-β1 pathway.Scientific reports · 2018Article
- CCR10+ epithelial cells from idiopathic pulmonary fibrosis lungs drive remodeling.JCI insight · 2018Article
- TGF-β1-induced deposition of provisional extracellular matrix by tracheal basal cells promotes epithelial-to-mesenchymal transition in a c-Jun NHAmerican journal of physiology. Lung cellular and molecular physiology · 2018Article
- TGF beta inhibits expression of SP-A, SP-B, SP-C, but not SP-D in human alveolar type II cells.Biochemical and biophysical research communications · 2018Article
- Potential contribution of alveolar epithelial type I cells to pulmonary fibrosis.Bioscience reports · 2017Review
- Lung Pericytes and Resident Fibroblasts: Busy Multitaskers.The American journal of pathology · 2016Review
- Role of CD248 as a potential severity marker in idiopathic pulmonary fibrosis.BMC pulmonary medicine · 2016Article
- Foxn1 Transcription Factor Regulates Wound Healing of Skin through Promoting Epithelial-Mesenchymal Transition.PloS one · 2016Article
- Astaxanthin prevents pulmonary fibrosis by promoting myofibroblast apoptosis dependent on Drp1-mediated mitochondrial fission.Journal of cellular and molecular medicine · 2015Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Epithelial-mesenchymal transition (EMT) has been considered to be involved in organ fibrogenesis. However, there is few direct evidence of this process in the pathophysiology of pulmonary fibrosis in vivo. Therefore, we tried to verify the involvement of this process in the development of pulmonary fibrosis. Since the co-expressions of epithelial and mesenchymal markers are thought to be a marker of EMT, we performed dual-immuunohistochemistry to assess the co-expressions of these proteins in lung tissues from bleomycin-induced pulmonary fibrosis in mice, and from patients with idiopathic pulmonary fibrosis, and nonspecific interstitial pneumonia. Double positive cells for epithelial markers including E-cadherin, T1alpha, or aquaporin 5, and a mesenchymal markers alpha-smooth muscle actin or vimentin were not found in bleomycin-induced pulmonary fibrosis in mice. Double positive cells for E-cadherin, ICAM-1, LEA, CD44v9, or SP-A and alpha-smooth muscle actin or vimentin were not found in lung tissues from normal lung parenchyma, idiopathic pulmonary fibrosis and nonspecific interstitial pneumonia. These results offer at least two possibilities. One is that EMT does not occur in IPF or bleomycin-induced pulmonary fibrosis in mice. Another is that EMT may occur in pulmonary fibrosis but the time during this transition in which cells express detectable levels of epithelial and mesenchymal markers is too small to be detected by double immunohistochemistry.
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