ArticlePLoS neglected tropical diseases2008
Transcriptional changes in the hookworm, Ancylostoma caninum, during the transition from a free-living to a parasitic larva.
Article in PLoS neglected tropical diseases, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 112 citations in OpenAlex.
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- Expression of Ascaris lumbricoides putative virulence-associated genes when infecting a human host.Parasites & vectors · 2021Article
- Twenty-five-year research progress in hookworm excretory/secretory products.Parasites & vectors · 2020Review
- Adaptive Radiation of the Flukes of the Family Fasciolidae Inferred from Genome-Wide Comparisons of Key Species.Molecular biology and evolution · 2020Article
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- Tissue-specific transcriptomes of Anisakis simplex (sensu stricto) and Anisakis pegreffii reveal potential molecular mechanisms involved in pathogenicity.Parasites & vectors · 2018Article
- Ancylostoma ceylanicum infective third-stage larvae are activated by co-culture with HT-29-MTX intestinal epithelial cells.Parasites & vectors · 2017Article
- Recombinant Haemonchus contortus 24 kDa excretory/secretory protein (rHcES-24) modulate the immune functions of goat PBMCs in vitro.Oncotarget · 2016Article
- CAP protein superfamily members in Toxocara canis.Parasites & vectors · 2016Article
- Expression profile of heat shock response factors during hookworm larval activation and parasitic development.Molecular and biochemical parasitology · 2015Article
- The structure of hookworm platelet inhibitor (HPI), a CAP superfamily member from Ancylostoma caninum.Acta crystallographica. Section F, Structural biology communications · 2015Article
- Apoplastic venom allergen-like proteins of cyst nematodes modulate the activation of basal plant innate immunity by cell surface receptors.PLoS pathogens · 2014Article
- Venom proteome of the box jellyfish Chironex fleckeri.PloS one · 2012Article
- Insights into SCP/TAPS proteins of liver flukes based on large-scale bioinformatic analyses of sequence datasets.PloS one · 2012Article
- RNA and protein synthesis is required for Ancylostoma caninum larval activation.Veterinary parasitology · 2011Article
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Authors and funding
8 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThird-stage larvae (L3) of the canine hookworm, Ancylostoma caninum, undergo arrested development preceding transmission to a host. Many of the mRNAs up-regulated at this stage are likely to encode proteins that facilitate the transition from a free-living to a parasitic larva. The initial phase of mammalian host invasion by A. caninum L3 (herein termed "activation") can be mimicked in vitro by culturing L3 in serum-containing medium. METHODOLOGY/PRINCIPAL
findingsThe mRNAs differentially transcribed between activated and non-activated L3 were identified by suppression subtractive hybridisation (SSH). The analysis of these mRNAs on a custom oligonucleotide microarray printed with the SSH expressed sequence tags (ESTs) and publicly available A. caninum ESTs (non-subtracted) yielded 602 differentially expressed mRNAs, of which the most highly represented sequences encoded members of the pathogenesis-related protein (PRP) superfamily and proteases. Comparison of these A. caninum mRNAs with those of Caenorhabditis elegans larvae exiting from developmental (dauer) arrest demonstrated unexpectedly large differences in gene ontology profiles. C. elegans dauer exiting L3 up-regulated expression of mostly intracellular molecules involved in growth and development. Such mRNAs are virtually absent from activated hookworm larvae, and instead are over-represented by mRNAs encoding extracellular proteins with putative roles in host-parasite interactions. CONCLUSIONS/SIGNIFICANCE: Although this should not invalidate C. elegans dauer exit as a model for hookworm activation, it highlights the limitations of this free-living nematode as a model organism for the transition of nematode larvae from a free-living to a parasitic state.
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