ReviewNature methods2008
Protein production and purification.
Review in Nature methods, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 305 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
305 citing papers in PubMed.
- AFM to Study Pore-Forming Proteins.Methods in molecular biology (Clifton, N.J.) · 2027Article
- A Novel Protein Recovery Method via Gel Separation and Silica Columns Capable of Rapid Antigen Purification and Deep Proteomic Identification.Molecules (Basel, Switzerland) · 2026Article
- An advanced LLM-powered article extraction pipeline delivers a database of buffers for protein studies and protein expression conditions.Briefings in bioinformatics · 2026Article
- Facile, high-throughput protein purification enabled by high-efficiency protease elution.Protein science : a publication of the Protein Society · 2026Article
- Efficient tag-less purification of recombinant human tau proteins.Analytical biochemistry · 2026Article
- A Miniaturized, High-Throughput Aqueous Solvent-Centric Method for Protein Solubility Screening.Biochemistry · 2026Article
- A katE katG double-knockout E. coli strain eliminates the risk of catalase contamination in recombinant proteins.Applied microbiology and biotechnology · 2026Article
- Construction and evaluation of an engineered autonomously lysing Salmonella system coupled with self-assembled nanoparticles as a vaccine platform for antigen delivery.Journal of nanobiotechnology · 2026Article
- A monofunctional-like mutant of DNA glycosylase NTHL1 changes the dynamics of DNA repair during acute oxidative stress.The Journal of biological chemistry · 2026Article
- Human cells for human proteins: Isotope labeling in mammalian cells in suspension for functional NMR studies.Protein science : a publication of the Protein Society · 2026Article
- RP3Net: a deep learning model for predicting recombinant protein production in Escherichia coli.Bioinformatics (Oxford, England) · 2026Article
- A biosensor toolbox for monitoring recombinant protein production inFrontiers in bioengineering and biotechnology · 2026Article
- Understanding the strengths and limitations of recombinant protein studies.Frontiers in molecular biosciences · 2026Review
- Detection of intramolecular protein dynamics on nanosecond-to-microsecond timescales by nanoparticle-assisted NMR spin relaxation (NASR).Nature protocols · 2026Review
- Article
- Construction of an ASFV proteome library via multiple optimization strategies for high-throughput analysis.Acta biochimica et biophysica Sinica · 2025Article
- Stabilising proteins in solution using affordable and readily available small molecules.Biophysical reviews · 2025Review
- Purification and Evaluation of the Biological Activity of Recombinant Mouse Prolactin.Journal of microbiology and biotechnology · 2025Article
- The C-terminal end of PLIN1 displays structural disorder.Biochemistry and biophysics reports · 2025Article
- Development of Recombinant Follicle-Stimulating Hormone for the Superovulation of Cattle: A Review.Veterinary sciences · 2025Review
245 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issuedHallberg, B Martin [added]
Authors and funding
87 authors.
Funding
Abstract
In selecting a method to produce a recombinant protein, a researcher is faced with a bewildering array of choices as to where to start. To facilitate decision-making, we describe a consensus 'what to try first' strategy based on our collective analysis of the expression and purification of over 10,000 different proteins. This review presents methods that could be applied at the outset of any project, a prioritized list of alternate strategies and a list of pitfalls that trip many new investigators.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.