ArticleJournal of clinical microbiology2008
Identification of Staphylococcus aureus and determination of methicillin resistance directly from positive blood cultures by isothermal amplification and a disposable detection device.
Article in Journal of clinical microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed.
- Isothermal nucleic acid amplification techniques: A comprehensive overview on their principle & applications as alternative to PCR.Practical laboratory medicine · 2026Review
- Recent advances and challenges of biosensing in point-of-care molecular diagnosis.Sensors and actuators. B, Chemical · 2021Review
- Developments in integrating nucleic acid isothermal amplification and detection systems for point-of-care diagnostics.Biosensors & bioelectronics · 2020Review
- Rapid Detection of Methicillin-ResistantDiagnostics (Basel, Switzerland) · 2020Review
- Current and Future Perspectives on Isothermal Nucleic Acid Amplification Technologies for Diagnosing Infections.Infection and drug resistance · 2020Review
- Detection of a microbial source tracking marker by isothermal helicase-dependent amplification and a nucleic acid lateral-flow strip test.Scientific reports · 2019Article
- Helicase-dependent isothermal amplification: a novel tool in the development of molecular-based analytical systems for rapid pathogen detection.Analytical and bioanalytical chemistry · 2018Review
- Evaluation of loop-mediated isothermal amplification for the rapid identification of bacteria and resistance determinants in positive blood cultures.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2017Article
- Implications of direct amplification for measuring antimicrobial resistance using point-of-care devices.Analytical methods : advancing methods and applications · 2017Article
- PCR-based Approaches for the Detection of Clinical Methicillin-resistant Staphylococcus aureus.The open microbiology journal · 2016Article
- Helicase-Dependent Isothermal Amplification of DNA and RNA by Using Self-Avoiding Molecular Recognition Systems.Chembiochem : a European journal of chemical biology · 2015Article
- Molecular-based isothermal tests for field diagnosis of malaria and their potential contribution to malaria elimination.The Journal of antimicrobial chemotherapy · 2015Review
- Article
- Low cost extraction and isothermal amplification of DNA for infectious diarrhea diagnosis.PloS one · 2013Article
- Detection of severe fever with thrombocytopenia syndrome virus by reverse transcription-cross-priming amplification coupled with vertical flow visualization.Journal of clinical microbiology · 2012Article
- Nucleic acid testing for tuberculosis at the point-of-care in high-burden countries.Expert review of molecular diagnostics · 2012Review
- Past, present and future molecular diagnosis and characterization of human immunodeficiency virus infections.Emerging microbes & infections · 2012Review
- Comparative clinical evaluation of the IsoAmp(®) HSV Assay with ELVIS(®) HSV culture/ID/typing test system for the detection of herpes simplex virus in genital and oral lesions.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2012Article
- A probe-based method for confirmation of methicillin-resistant Staphylococcus aureus and detection of Panton-Valentine leukocidin and tst virulence genes.Diagnostic microbiology and infectious disease · 2011Article
- Instrument-free nucleic acid amplification assays for global health settings.Proceedings of SPIE--the International Society for Optical Engineering · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
A simple, rapid, and user-friendly procedure has been developed to identify Staphylococcus aureus and determine its methicillin resistance directly from gram-positive cocci in cluster-containing blood culture medium. The specimens were diluted and heated prior to amplification of the nuc and mecA genes with isothermal helicase-dependent amplification. Amplicons were detected using a disposable detection device. The analytical sensitivity of the assays was 50 CFU per reaction, and the clinical sensitivity and specificity were both 100% for S. aureus detection and 100% and 98% for methicillin resistance determination, respectively.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.