ArticleJournal of clinical microbiology2008
Rapid identification and differentiation of Trichophyton species, based on sequence polymorphisms of the ribosomal internal transcribed spacer regions, by rolling-circle amplification.
Article in Journal of clinical microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed.
- Prevalence of dermatophytosis in cats and dogs in türkiye: dominance of Microsporum canis and first detection of Trichophyton rubrum.BMC veterinary research · 2025Article
- Emerging Human Health Problems Caused by Pathogenic and Immuno-activating Fungi.Current topics in microbiology and immunology · 2025Review
- Article
- Article
- Dermatophytosis Caused by Trichophyton indotineae (Trichophyton mentagrophytes ITS Genotype VIII) in Sri Lanka.The American journal of tropical medicine and hygiene · 2024Article
- Review
- The epidemic of the multiresistant dermatophyteFrontiers in immunology · 2022Article
- A simple multiplex polymerase chain reaction assay for rapid identification of the common pathogenic dermatophytes: Trichophyton interdigitale, Trichophyton rubrum, and Epidermophyton floccosum.Current medical mycology · 2021Article
- Selective Sweeps Uncovering the Genetic Basis of Horn and Adaptability Traits on Fine-Wool Sheep in China.Frontiers in genetics · 2021Article
- Article
- Lineages Within the Trichophyton rubrum Complex.Mycopathologia · 2020Article
- Identification of Mycoses in Developing Countries.Journal of fungi (Basel, Switzerland) · 2019Review
- Rapid Identification of Seven Waterborne Exophiala Species by RCA DNA Padlock Probes.Mycopathologia · 2018Article
- A severe transmissible Majocchi's granuloma in an immunocompetent returned traveler.Medical mycology case reports · 2017Article
- Use of Padlock Probes and Rolling Circle Amplification (RCA) for Rapid Identification of Trichophyton Species, Related to Human and Animal Disorder.Jundishapur journal of microbiology · 2015Article
- Rapid Identification of Emerging Human-Pathogenic Sporothrix Species with Rolling Circle Amplification.Frontiers in microbiology · 2015Article
- Rapid identification of black grain eumycetoma causative agents using rolling circle amplification.PLoS neglected tropical diseases · 2014Article
- Restriction analysis of β-tubulin gene for differentiation of the common pathogenic dermatophytes.Journal of clinical laboratory analysis · 2014Article
- Use of rolling circle amplification to rapidly identify species of Cladophialophora potentially causing human infection.Mycopathologia · 2013Article
- Practical identification of eight medically important Trichosporon species by reverse line blot hybridization (RLB) assay and rolling circle amplification (RCA).Medical mycology · 2013Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA sequencing analyses have demonstrated relatively limited polymorphisms within the fungal internal transcribed spacer (ITS) regions among Trichophyton spp. We sequenced the ITS region (ITS1, 5.8S, and ITS2) for 42 dermatophytes belonging to seven species (Trichophyton rubrum, T. mentagrophytes, T. soudanense, T. tonsurans, Epidermophyton floccosum, Microsporum canis, and M. gypseum) and developed a novel padlock probe and rolling-circle amplification (RCA)-based method for identification of single nucleotide polymorphisms (SNPs) that could be exploited to differentiate between Trichophyton spp. Sequencing results demonstrated intraspecies genetic variation for T. tonsurans, T. mentagrophytes, and T. soudanense but not T. rubrum. Signature sets of SNPs between T. rubrum and T. soudanense (4-bp difference) and T. violaceum and T. soudanense (3-bp difference) were identified. The RCA assay correctly identified five Trichophyton species. Although the use of two "group-specific" probes targeting both the ITS1 and the ITS2 regions were required to identify T. soudanense, the other species were identified by single ITS1- or ITS2-targeted species-specific probes. There was good agreement between ITS sequencing and the RCA assay. Despite limited genetic variation between Trichophyton spp., the sensitive, specific RCA-based SNP detection assay showed potential as a simple, reproducible method for the rapid (2-h) identification of Trichophyton spp.
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