ArticlePLoS biology2008
Inhibitory phosphorylation of separase is essential for genome stability and viability of murine embryonic germ cells.
Article in PLoS biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 44 citations in OpenAlex.
- Review
- MicroRNA-202 safeguards meiotic progression by preventing premature SEPARASE-mediated REC8 cleavage.EMBO reports · 2022Article
- Proteolysis in Reproduction: Lessons From Gene-Modified Organism Studies.Frontiers in endocrinology · 2022Review
- Genome diversity and instability in human germ cells and preimplantation embryos.Seminars in cell & developmental biology · 2021Article
- Intertwined Functions of Separase and Caspase in Cell Division and Programmed Cell Death.Scientific reports · 2020Article
- Article
- The ubiquitin ligase CRL2ZYG11 targets cyclin B1 for degradation in a conserved pathway that facilitates mitotic slippage.The Journal of cell biology · 2016Article
- Positive and negative regulation of vertebrate separase by Cdk1-cyclin B1 may explain why securin is dispensable.The Journal of biological chemistry · 2015Article
- The biochemistry of mitosis.Cold Spring Harbor perspectives in biology · 2015Review
- Overexpression and constitutive nuclear localization of cohesin protease Separase protein correlates with high incidence of relapse and reduced overall survival in glioblastoma multiforme.Journal of neuro-oncology · 2014Article
- PP2A delays APC/C-dependent degradation of separase-associated but not free securin.The EMBO journal · 2014Article
- Pttg1/securin is required for the branching morphogenesis of the mammary gland and suppresses mammary tumorigenesis.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Bub1 kinase activity drives error correction and mitotic checkpoint control but not tumor suppression.The Journal of cell biology · 2012Article
- The sister bonding of duplicated chromosomes.Seminars in cell & developmental biology · 2011Review
- Separase phosphosite mutation leads to genome instability and primordial germ cell depletion during oogenesis.PloS one · 2011Article
- Article
- Loss of spindle assembly checkpoint-mediated inhibition of Cdc20 promotes tumorigenesis in mice.The Journal of cell biology · 2009Article
- Overexpression and mislocalization of the chromosomal segregation protein separase in multiple human cancers.Clinical cancer research : an official journal of the American Association for Cancer Research · 2009Article
- Preimplantation mouse embryos depend on inhibitory phosphorylation of separase to prevent chromosome missegregation.Molecular and cellular biology · 2009Article
- DNA-dependent cohesin cleavage by separase.Nucleus (Austin, Tex.)Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Activity of separase, a cysteine protease that cleaves sister chromatid cohesin at the onset of anaphase, is tightly regulated to ensure faithful chromosome segregation and genome stability. Two mechanisms negatively regulate separase: inhibition by securin and phosphorylation on serine 1121. To gauge the physiological significance of the inhibitory phosphorylation, we created a mouse strain in which Ser1121 was mutated to Ala (S1121A). Here we report that this S1121A point mutation causes infertility in mice. We show that germ cells in the mutants are depleted during development. We further demonstrate that S1121A causes chromosome misalignment during proliferation of the postmigratory primordial germ cells, resulting in mitotic arrest, aneuploidy, and eventual cell death. Our results indicate that inhibitory phosphorylation of separase plays a critical role in the maintenance of sister chromatid cohesion and genome stability in proliferating postmigratory primordial germ cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.