ArticlePflugers Archiv : European journal of physiology2008
Softening of the actin cytoskeleton by inhibition of myosin II.
Article in Pflugers Archiv : European journal of physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed.
- Measuring the Elastic Properties of Living Cells.Methods in molecular biology (Clifton, N.J.) · 2027Article
- CFM: confinement force microscopy-a dynamic, precise and stable microconfiner for traction force microscopy in spatial confinement.Nature methods · 2026Article
- Mechanochemical interactions in cancer cells: The role of substrate stiffness in cell behavior and drug response.PloS one · 2026Article
- Hydrogel-Mediated Preservation of Live Tumor Explants for Drug Development in Peritoneal Metastases.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Robust quantification of cellular mechanics using optical tweezers.Biophysical reports · 2025Article
- A mechanical transition from tension to buckling underlies the jigsaw puzzle shape morphogenesis of histoblasts in the Drosophila epidermis.PLoS biology · 2024Article
- How cytoskeletal crosstalk makes cells move: Bridging cell-free and cell studies.Biophysics reviews · 2024Review
- Bioelectric stimulation controls tissue shape and size.Nature communications · 2024Article
- Acoustic Wave-Induced Stroboscopic Optical Mechanotyping of Adherent Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Asymmetrical positioning of cell organelles reflects the cell chirality of mouse myoblast cells.APL bioengineering · 2024Article
- Intracellular tension sensor reveals mechanical anisotropy of the actin cytoskeleton.Nature communications · 2023Article
- Evidence that tissue recoil in the earlyMolecular biology of the cell · 2023Article
- The effects of measurement parameters on the cancerous cell nucleus characterisation by atomic force microscopy in vitro.Journal of microscopy · 2022Article
- Transparent, Compliant 3D Mesostructures for Precise Evaluation of Mechanical Characteristics of Organoids.Advanced materials (Deerfield Beach, Fla.) · 2021Article
- Encoding memory in tube diameter hierarchy of living flow network.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Qualitative analysis of contribution of intracellular skeletal changes to cellular elasticity.Cellular and molecular life sciences : CMLS · 2020Review
- Cell Mechanical and Physiological Behavior in the Regime of Rapid Mechanical Compressions that Lead to Cell Volume Change.Small (Weinheim an der Bergstrasse, Germany) · 2020Article
- Nano-mechanical mapping of interdependent cell and ECM mechanics by AFM force spectroscopy.Scientific reports · 2019Article
- Article
- Evaluation of the elastic Young's modulus and cytotoxicity variations in fibroblasts exposed to carbon-based nanomaterials.Journal of nanobiotechnology · 2019Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have investigated the mechanical properties of fibroblast cells after adding the myosin inhibitor blebbistatin and the Rho-kinase inhibitor Y-27632 by atomic force microscopy (AFM). We have observed a decrease in the elastic modulus from a value of around 20 kPa down to a value around 8 kPa on a time scale of around 30-60 min when applying the myosin inhibitor blebbistatin, whereas the Y-27632 did not show any prominent mechanical effects. From topographic images, we can conclude that, after adding blebbistatin, actin filaments are not visible any more, whereas Y-27632 did not show any prominent effects in cell morphology. This study shows that tension generated by myosin contributes to the cellular stiffness and thus can be observed by measuring the elastic modulus of cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.