ArticlePloS one2008
A biomedically enriched collection of 7000 human ORF clones.
Article in PloS one, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Site-specific recombinational cloning using gateway and in-fusion cloning schemes.Current protocols in molecular biology · 2015Article
- Exploration of panviral proteome: high-throughput cloning and functional implications in virus-host interactions.Theranostics · 2014Article
- A plasmid toolkit for cloning chimeric cDNAs encoding customized fusion proteins into any Gateway destination expression vector.BMC molecular biology · 2013Article
- Review
- Beyond hairballs: The use of quantitative mass spectrometry data to understand protein-protein interactions.FEBS letters · 2012Review
- PSI:Biology-materials repository: a biologist's resource for protein expression plasmids.Journal of structural and functional genomics · 2011Article
- A public genome-scale lentiviral expression library of human ORFs.Nature methods · 2011Article
- New technologies in cancer. Protein microarrays for biomarker discovery.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2011Review
- Nucleic acid programmable protein array a just-in-time multiplexed protein expression and purification platform.Methods in enzymology · 2011Article
- Online nanoflow RP-RP-MS reveals dynamics of multicomponent Ku complex in response to DNA damage.Journal of proteome research · 2010Article
- Protein Structure Initiative Material Repository: an open shared public resource of structural genomics plasmids for the biological community.Nucleic acids research · 2010Article
- Target identification using drug affinity responsive target stability (DARTS).Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Biomedical text mining and its applications.PLoS computational biology · 2009Article
- Proteomic analysis of the hyaloid vascular system regression during ocular development.Journal of proteome research · 2008Article
- Applications of protein microarrays for biomarker discovery.Proteomics. Clinical applications · 2008Article
- Next-generation high-density self-assembling functional protein arrays.Nature methods · 2008Article
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Authors and funding
18 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report the production and availability of over 7000 fully sequence verified plasmid ORF clones representing over 3400 unique human genes. These ORF clones were derived using the human MGC collection as template and were produced in two formats: with and without stop codons. Thus, this collection supports the production of either native protein or proteins with fusion tags added to either or both ends. The template clones used to generate this collection were enriched in three ways. First, gene redundancy was removed. Second, clones were selected to represent the best available GenBank reference sequence. Finally, a literature-based software tool was used to evaluate the list of target genes to ensure that it broadly reflected biomedical research interests. The target gene list was compared with 4000 human diseases and over 8500 biological and chemical MeSH classes in approximately 15 Million publications recorded in PubMed at the time of analysis. The outcome of this analysis revealed that relative to the genome and the MGC collection, this collection is enriched for the presence of genes with published associations with a wide range of diseases and biomedical terms without displaying a particular bias towards any single disease or concept. Thus, this collection is likely to be a powerful resource for researchers who wish to study protein function in a set of genes with documented biomedical significance.
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