ArticlePloS one2008
A novel ZAP-70 dependent FRET based biosensor reveals kinase activity at both the immunological synapse and the antisynapse.
Article in PloS one, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 42 citations in OpenAlex.
- LAT condensation gates PLCγ1 activation via bimodal LAT phosphorylation.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Molecular Spies in Action: Genetically Encoded Fluorescent Biosensors Light up Cellular Signals.Chemical reviews · 2024Review
- Neo-Antigen-Reactive T Cells Immunotherapy for Colorectal Cancer: A More Personalized Cancer Therapy Approach.Global challenges (Hoboken, NJ) · 2023Review
- Optical sensing and control of T cell signaling pathways.Frontiers in physiology · 2023Review
- Integration of FRET and sequencing to engineer kinase biosensors from mammalian cell libraries.Nature communications · 2021Article
- Bioengineering tools for probing intracellular events in T lymphocytes.WIREs mechanisms of disease · 2021Review
- Review
- Dynamic Mitochondrial Migratory Features Associated with Calcium Responses during T Cell Antigen Recognition.Journal of immunology (Baltimore, Md. : 1950) · 2019Article
- Genetically Encoded Fluorescent Biosensors Illuminate the Spatiotemporal Regulation of Signaling Networks.Chemical reviews · 2018Review
- Imaging Spatiotemporal Activities of ZAP-70 in Live T Cells Using a FRET-Based Biosensor.Annals of biomedical engineering · 2016Article
- Fluorescent Reporters and Biosensors for Probing the Dynamic Behavior of Protein Kinases.Proteomes · 2015Review
- Fluorescent proteins as genetically encoded FRET biosensors in life sciences.Sensors (Basel, Switzerland) · 2015Review
- Biosensor architectures for high-fidelity reporting of cellular signaling.Biophysical journal · 2014Article
- Developments in preclinical cancer imaging: innovating the discovery of therapeutics.Nature reviews. Cancer · 2014Review
- Regulation of ras exchange factors and cellular localization of ras activation by lipid messengers in T cells.Frontiers in immunology · 2013Review
- Application of FRET probes in the analysis of neuronal plasticity.Frontiers in neural circuits · 2013Review
- Murine Tim-1 is excluded from the immunological synapse.F1000Research · 2012Article
- A FRET-Based Biosensor for Imaging SYK Activities in Living Cells.Cellular and molecular bioengineering · 2011Article
- Imaging techniques for assaying lymphocyte activation in action.Nature reviews. Immunology · 2011Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many hypotheses attempting to explain the speed and sensitivity with which a T-cell discriminates the antigens it encounters include a notion of relative spatial and temporal control of particular biochemical steps involved in the process. An essential step in T-cell receptor (TCR) mediated signalling is the activation of the protein tyrosine kinase ZAP-70. ZAP-70 is recruited to the TCR upon receptor engagement and, once activated, is responsible for the phosphorylation of the protein adaptor, Linker for Activation of T-cells, or LAT. LAT phosphorylation results in the recruitment of a signalosome including PLCgamma1, Grb2/SOS, GADS and SLP-76. In order to examine the real time spatial and temporal evolution of ZAP-70 activity following TCR engagement in the immune synapse, we have developed ROZA, a novel FRET-based biosensor whose function is dependent upon ZAP-70 activity. This new probe not only provides a measurement of the kinetics of ZAP-70 activity, but also reveals the subcellular localization of the activity as well. Unexpectedly, ZAP-70 dependent FRET was observed not only at the T-cell -APC interface, but also at the opposite pole of the cell or "antisynapse".
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.