ArticlePloS one2008
Coronavirus spike protein inhibits host cell translation by interaction with eIF3f.
Article in PloS one, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 79 citations in OpenAlex.
- Enteral delivery of proteins enhances the expression of proteins involved in the cytoskeleton and protein biosynthesis in human duodenal mucosa.The American journal of clinical nutrition · 2015Trial
- Coronavirus endoribonuclease nsp15 suppresses host protein synthesis and evades PKR-eIF2α-mediated translation shutoff to ensure viral protein synthesis.PLoS pathogens · 2025Article
- Dissecting infectious bronchitis virus-induced host shutoff at the translation level.Journal of virology · 2024Article
- Machine Learning to Identify Critical Biomarker Profiles in New SARS-CoV-2 Variants.Microorganisms · 2024Article
- SARS-CoV-2 viral protein Nsp2 stimulates translation under normal and hypoxic conditions.Virology journal · 2023Article
- Prospective Roles of Tumor Necrosis Factor-Alpha (TNF-α) in COVID-19: Prognosis, Therapeutic and Management.International journal of molecular sciences · 2023Review
- SARS-CoV-2 Harnesses Host Translational Shutoff and Autophagy To Optimize Virus Yields: the Role of the Envelope (E) Protein.Microbiology spectrum · 2023Article
- A novel logical model of COVID-19 intracellular infection to support therapies development.PLoS computational biology · 2022Article
- Drug repurposing for COVID-19 using computational screening: Is Fostamatinib/R406 a potential candidate?Methods (San Diego, Calif.) · 2022Article
- The Evolutionary Dance between Innate Host Antiviral Pathways and SARS-CoV-2.Pathogens (Basel, Switzerland) · 2022Review
- Prevention, treatment and potential mechanism of herbal medicine for Corona viruses: A review.Bioengineered · 2022Review
- Ozone exposure upregulates the expression of host susceptibility protein TMPRSS2 to SARS-CoV-2.Scientific reports · 2022Article
- Translational Control of COVID-19 and Its Therapeutic Implication.Frontiers in immunology · 2022Review
- Systematic analysis of SARS-CoV-2 infection of an ACE2-negative human airway cell.Cell reports · 2021Article
- Translational shutdown and evasion of the innate immune response by SARS-CoV-2 NSP14 protein.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Molecular Regulation of Skeletal Muscle Growth and Organelle Biosynthesis: Practical Recommendations for Exercise Training.International journal of molecular sciences · 2021Review
- Systematic analysis of SARS-CoV-2 infection of an ACE2-negative human airway cell.bioRxiv : the preprint server for biology · 2021Article
- Roadblocks and fast tracks: How RNA binding proteins affect the viral RNA journey in the cell.Seminars in cell & developmental biology · 2021Review
- SARS-CoV-2 innate effector associations and viral load in early nasopharyngeal infection.Physiological reports · 2021Observational
- The chemical biology of coronavirus host-cell interactions.RSC chemical biology · 2021Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In response to viral infection, the expression of numerous host genes, including predominantly a number of proinflammatory cytokines and chemokines, is usually up-regulated at both transcriptional and translational levels. It was noted that in cells infected with coronavirus, transcription and translation of some of these genes were differentially induced. Drastic induction of their expression at the transcriptional level was observed in cells infected with coronavirus. However, induction of the same genes at the translational level was usually found to be minimal to moderate. To investigate the underlying mechanisms, yeast two-hybrid screen was carried out using SARS-CoV proteins as baits, revealing that a subunit of the eukaryotic initiation factor 3 (eIF3), eIF3f, may interact with the N-terminal region of the SARS-CoV spike (S) protein. This interaction was subsequently confirmed by co-immunoprecipitation and immunofluorescent staining. Meanwhile, parallel experiments confirmed that eIF3f could also interact with the S protein of another coronavirus, the avian coronavirus infectious bronchitis virus (IBV). These interactions led to the inhibition of translation of a reporter gene in both in vitro expression system and intact cells. Interestingly, IBV-infected cells stably expressing a Flag-tagged eIF3f showed much higher translation of IL-6 and IL-8, suggesting that the interaction between coronavirus S protein and eIF3f plays a functional role in controlling the expression of host genes, especially genes that are induced during coronavirus infection cycles. This study reveals a novel mechanism exploited by coronavirus to regulate viral pathogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.