Evidence map›Paper›PMID 18231571›Full record

ArticleSensors and actuators. B, Chemical2007

Antimicrobial peptides as new recognition molecules for screening challenging species.

Nadezhda V Kulagina, Kara M Shaffer, Frances S Ligler, Chris R Taitt

Abstract read
In one paragraph

Article in Sensors and actuators. B, Chemical, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
3.2field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 70 citations in OpenAlex.

  1. Review
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  11. Application of immobilized synthetic anti-lipopolysaccharide peptides for the isolation and detection of bacteria.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2015
    Article
  12. Improved bacterial detection using immobilized acyl-lysyl oligomers.Applied and environmental microbiology · 2015
    Article
  13. Review
  14. Article
  15. Review
  16. Review
  17. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Nadezhda V KulaginaCenter for Bio/Molecular Science & Engineering, Naval Research Laboratory, Washington DC, 20375-5348 USA.
Kara M Shaffer
Frances S Ligler
Chris R Taitt
United States Naval Research Laboratory · USNaval Research Laboratory Center for Bio/Molecular Science and Engineering

Funding

Arrays of Receptors for Infectious Disease DiagnosisR01EB000680 · NIBIB · U.S. NAVAL RESEARCH LABORATORY · PI LIGLER, FRANCES S. · 2003 to 2005
$1.8M
NIBIB NIH HHS R01 EB000680
6 · The paper itself

Abstract

The goal of this study was to evaluate binding of four targets of biodefense interest to immobilized antimicrobial peptides (AMPs) in biosensor assays. Polymyxins B and E, melittin, cecropins A, B, and P, parasin, bactenecin and magainin-1, as well as control antibodies, were used as capture molecules for detection of Cy3-labeled Venezuelan equine encephalitis virus (VEE), vaccinia virus, C. burnetti and B. melitensis. Although VEE, vaccinia virus and C. burnetti did not show any binding activity to their corresponding capture antibodies, B. melitensis bound to immobilized anti-Brucella monoclonal antibodies. The majority of the immobilized AMPs included in this study bound labeled VEE, vaccinia virus and C. burnetti in a concentration-dependent manner, and B. melitensis bound to polymyxin B, polymyxin E, and bactenecin. No binding was observed on immobilized magainin-1. In contrast to all bacterial targets tested to date, VEE and vaccinia virus demonstrated similar patterns of binding to all peptides. While the direct assay is generally replaced by a sandwich assay for analysis of real-world samples, direct binding experiments are commonly used to characterize specificity and sensitivity of binding molecules. In this case, they clearly demonstrate the capability of AMPs as recognition molecules for four biothreat agents.

Identifiers

PMID18231571
PMCPMC1826611
OpenAlexW2009952797

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.