ArticleGenes & development2008
STAR family RNA-binding protein ASD-2 regulates developmental switching of mutually exclusive alternative splicing in vivo.
Article in Genes & development, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers, 1 of them a synthesis that pooled it.
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Who cites it
40 citing papers in PubMed, 1 synthesis or guideline pooled it, 80 citations in OpenAlex.
- Quantitative RNA-seq meta-analysis of alternative exon usage inGenome research · 2017Pooled it
- Competition for the conserved branch point sequence influences physiological outcomes in pre-mRNA splicing.eLife · 2026Article
- An updated compendium of Caenorhabditis elegans RNA-binding proteins and their regulation.G3 (Bethesda, Md.) · 2025Article
- Alternative splicing across the C. elegans nervous system.Nature communications · 2025Article
- Review
- Definition of germ layer cell lineage alternative splicing programs reveals a critical role for Quaking in specifying cardiac cell fate.Nucleic acids research · 2022Article
- KH domain containing RNA-binding proteins coordinate with microRNAs to regulate Caenorhabditis elegans development.G3 (Bethesda, Md.) · 2021Article
- mRNA Editing, Processing and Quality Control inGenetics · 2020Review
- Deterministic splicing ofScience advances · 2019Article
- Alternative splicing of the Caenorhabditis elegans lev-11 tropomyosin gene is regulated in a tissue-specific manner.Cytoskeleton (Hoboken, N.J.) · 2018Article
- Post-Transcriptional Regulation of Anti-Apoptotic BCL2 Family Members.International journal of molecular sciences · 2018Review
- The combinatorial control of alternative splicing in C. elegans.PLoS genetics · 2017Article
- Evolutionary Dynamics of GLD-1-mRNA complexes in Caenorhabditis nematodes.Genome biology and evolution · 2014Article
- RBFOX and SUP-12 sandwich a G base to cooperatively regulate tissue-specific splicing.Nature structural & molecular biology · 2014Article
- Formation of nuclear bodies by the lncRNA Gomafu-associating proteins Celf3 and SF1.Genes to cells : devoted to molecular & cellular mechanisms · 2014Article
- A pair of RNA-binding proteins controls networks of splicing events contributing to specialization of neural cell types.Molecular cell · 2014Article
- The Drosophila RNA-binding protein HOW controls the stability of dgrasp mRNA in the follicular epithelium.Nucleic acids research · 2014Article
- Article
- Article
- Quaking and PTB control overlapping splicing regulatory networks during muscle cell differentiation.RNA (New York, N.Y.) · 2013Article
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3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Alternative splicing of pre-mRNAs greatly contributes to the spatiotemporal diversity of gene expression in metazoans. However, the molecular basis of developmental regulation and the precise sequence of alternative pre-mRNA processing in vivo are poorly understood. In the present study, we focus on the developmental switching of the mutually exclusive alternative splicing of the let-2 gene of Caenorhabditis elegans from the exon 9 form in embryos to the exon 10 form in adults. By visualizing the usage of the let-2 mutually exclusive exons through differential expression of green fluorescent protein (GFP) and red fluorescent protein (RFP), we isolated several switching-defective mutants and identified the alternative splicing defective-2 (asd-2) gene, encoding a novel member of the evolutionarily conserved STAR (signal transduction activators of RNA) family of RNA-binding proteins. Comparison of the amounts of partially spliced let-2 RNAs in synchronized wild-type and asd-2 mutant worms suggested that either of the introns downstream from the let-2 mutually exclusive exons is removed prior to the removal of the upstream ones, and that asd-2 promotes biased excision of intron 10 in the late larval stages. We propose that the developmental switching between alternative sequences of intron removal determines the ratio between the mature let-2 mRNA isoforms.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.