ArticleBMC clinical pathology2008
Preservation of biomolecules in breast cancer tissue by a formalin-free histology system.
Article in BMC clinical pathology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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Who cites it
13 citing papers in PubMed, 58 citations in OpenAlex.
- Glyoxal acid-free (GAF) histological fixative is a suitable alternative to formalin: results from an open-label comparative non-inferiority study.Virchows Archiv : an international journal of pathology · 2024Article
- Effects of short and long-term alcohol-based fixation on Sprague-Dawley rat tissue morphology, protein and nucleic acid preservation.Acta histochemica · 2019Article
- Methanol-based fixation is superior to buffered formalin for next-generation sequencing of DNA from clinical cancer samples.Annals of oncology : official journal of the European Society for Medical Oncology · 2016Article
- Tissue fixation and the effect of molecular fixatives on downstream staining procedures.Methods (San Diego, Calif.) · 2014Review
- Molecular fixative enables expression microarray analysis of microdissected clinical cervical specimens.Experimental and molecular pathology · 2014Article
- Comparison of routine fixation of tissues with rapid tissue fixation.Journal of clinical and diagnostic research : JCDR · 2013Article
- Quantitation of fixative-induced morphologic and antigenic variation in mouse and human breast cancers.Laboratory investigation; a journal of technical methods and pathology · 2013Article
- Systematic comparison of tissue fixation with alternative fixatives to conventional tissue fixation with buffered formalin in a xenograft-based model.Virchows Archiv : an international journal of pathology · 2012Article
- Quantitative analysis of p53 expression in human normal and cancer tissue microarray with global normalization method.International journal of clinical and experimental pathology · 2011Article
- Phosphoprotein stability in clinical tissue and its relevance for reverse phase protein microarray technology.Methods in molecular biology (Clifton, N.J.) · 2011Article
- Reverse phase protein microarrays advance to use in clinical trials.Molecular oncology · 2010Review
- Tissue is alive: New technologies are needed to address the problems of protein biomarker pre-analytical variability.Proteomics. Clinical applications · 2009Article
- Immunohistochemical detection of estrogen and progesterone receptor and HER2 expression in breast carcinomas: comparison of cell block and tissue block preparations.International journal of clinical and experimental pathology · 2009Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe potential problems associated with the use of formalin in histology, such as health hazards, degradation of RNA and cross-linking of proteins are well recognized. We describe the utilization of a formalin-free fixation and processing system for tissue detection of two important biopredictors in breast cancer - estrogen receptor and HER2 - at the RNA and protein levels.
methodsParallel sections of 62 cases of breast cancer were fixed in an alcohol-based molecular fixative and in formalin. Molecular fixative samples were processed by a novel formalin-free microwave-assisted processing system that preserves DNA, RNA and proteins. Formalin-fixed samples were processed using the conventional method. Estrogen receptor was assessed by immunohistochemistry and real-time PCR. HER2 was assessed by immunohistochemistry, FISH, CISH and real-time PCR.
resultsThe immunohistochemical reaction for estrogen receptor was similar in molecular- and formalin-fixed samples (Spearman Rank R = 0.83, p < 0.05). Also HER2 result was similar to that of formalin-fixed counterparts after elimination of antigen retrieval step (Spearman Rank R = 0.84, p < 0.05). The result of HER2 amplification by FISH and CISH was identical in the molecular fixative and formalin-fixed samples; although a shorter digestion step was required when using the former fixative. Real-time PCR for both estrogen receptor and HER2 were successful in all of the molecular fixative specimens.
conclusionThe formalin-free tissue fixation and processing system is a practical platform for evaluation of biomolecular markers in breast cancer and it allows reliable DNA and RNA and protein studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.