ArticleBMC genomics2008
Genome-wide transcriptome analysis reveals that a pleiotropic antibiotic regulator, AfsS, modulates nutritional stress response in Streptomyces coelicolor A3(2).
Article in BMC genomics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 80 citations in OpenAlex.
- Optimized production of concanamycins using a rational metabolic engineering strategy.Metabolic engineering · 2025Article
- Structural and functional characterization of AfsR, an SARP family transcriptional activator of antibiotic biosynthesis in Streptomyces.PLoS biology · 2024Article
- Small molecule inducers of actinobacteria natural product biosynthesis.Journal of industrial microbiology & biotechnology · 2023Review
- Biomass-derived molecules modulate the behavior of Streptomyces coelicolor for antibiotic production.3 Biotech · 2016Article
- Cyclic diguanylate signaling in Gram-positive bacteria.FEMS microbiology reviews · 2016Review
- ScbR- and ScbR2-mediated signal transduction networks coordinate complex physiological responses in Streptomyces coelicolor.Scientific reports · 2015Article
- Deciphering the regulon of Streptomyces coelicolor AbrC3, a positive response regulator of antibiotic production.Applied and environmental microbiology · 2014Article
- Activating secondary metabolism with stress and chemicals.Journal of industrial microbiology & biotechnology · 2014Review
- Synthetic promoter library for modulation of actinorhodin production in Streptomyces coelicolor A3(2).PloS one · 2014Article
- Systems perspectives on erythromycin biosynthesis by comparative genomic and transcriptomic analyses of S. erythraea E3 and NRRL23338 strains.BMC genomics · 2013Article
- Molecular regulation of antibiotic biosynthesis in streptomyces.Microbiology and molecular biology reviews : MMBR · 2013Review
- Identification and biotechnological application of novel regulatory genes involved in Streptomyces polyketide overproduction through reverse engineering strategy.BioMed research international · 2013Article
- An insight into the "-omics" based engineering of streptomycetes for secondary metabolite overproduction.BioMed research international · 2013Review
- Article
- Genomic and transcriptomic insights into the thermo-regulated biosynthesis of validamycin in Streptomyces hygroscopicus 5008.BMC genomics · 2012Article
- Extracting regulator activity profiles by integration of de novo motifs and expression data: characterizing key regulators of nutrient depletion responses in Streptomyces coelicolor.Nucleic acids research · 2012Article
- Intracellular Metabolite Pool Changes in Response to Nutrient Depletion Induced Metabolic Switching in Streptomyces coelicolor.Metabolites · 2012Article
- Article
- Signals and regulators that govern Streptomyces development.FEMS microbiology reviews · 2012Review
- The -omics Era- Toward a Systems-Level Understanding of Streptomyces.Current genomics · 2011Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundA small "sigma-like" protein, AfsS, pleiotropically regulates antibiotic biosynthesis in Streptomyces coelicolor. Overexpression of afsS in S. coelicolor and certain related species causes antibiotic stimulatory effects in the host organism. Although recent studies have uncovered some of the upstream events activating this gene, the mechanisms through which this signal is relayed downstream leading to the eventual induction of antibiotic pathways remain unclear.
resultsIn this study, we employed whole-genome DNA microarrays and quantitative PCRs to examine the transcriptome of an afsS disruption mutant that is completely deficient in the production of actinorhodin, a major S. coelicolor antibiotic. The production of undecylprodigiosin, another prominent antibiotic, was, however, perturbed only marginally in the mutant. Principal component analysis of temporal gene expression profiles identified two major gene classes each exhibiting a distinct coordinate differential expression pattern. Surprisingly, nearly 70% of the >117 differentially expressed genes were conspicuously associated with nutrient starvation response, particularly those of phosphate, nitrogen and sulfate. Furthermore, expression profiles of some transcriptional regulators including at least two sigma factors were perturbed in the mutant. In almost every case, the effect of afsS disruption was not observed until the onset of stationary phase.
conclusionOur data suggests a comprehensive role for S. coelicolor AfsS as a master regulator of both antibiotic synthesis and nutritional stress response, reminiscent of alternative sigma factors found in several bacteria.
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