ArticleBMC genomics2008
Identification of protein secretion systems and novel secreted proteins in Rhizobium leguminosarum bv. viciae.
Article in BMC genomics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 77 citations in OpenAlex.
- Comparative Genomic Analysis of the Type VI Secretion System in Strains of the Rhizobium leguminosarum Species Complex with Variable Host Specificity.Current microbiology · 2026Article
- Rhizobial Nod factors modulate reactive oxygen species, jasmonates, and pattern-recognizing receptors to suppress immune response.Plant molecular biology · 2026Article
- Characterization and De Novo Genome Assembly for New Rhizobium Ruizarguesonis Rhizobial Strain Vst36-3 Involved in Symbiosis with Pisum and Vicia Plants.Current microbiology · 2025Article
- Article
- Secretory molecules from secretion systems fine-tune the host-beneficial bacteria (PGPRs) interaction.Frontiers in microbiology · 2024Review
- NopAA and NopD Signaling Association-Related GeneInternational journal of molecular sciences · 2023Article
- Impact of c-di-GMP on the Extracellular Proteome ofBiology · 2022Article
- RapD Is a Multimeric Calcium-Binding Protein That Interacts With theFrontiers in microbiology · 2022Article
- Article
- Rhizobial Exopolysaccharides: Genetic Regulation of Their Synthesis and Relevance in Symbiosis with Legumes.International journal of molecular sciences · 2021Review
- Structure and Development of the Legume-Rhizobial Symbiotic Interface in Infection Threads.Cells · 2021Review
- Rhizobial Chemotaxis and Motility Systems at Work in the Soil.Frontiers in plant science · 2021Review
- Lifestyle adaptations ofProceedings of the National Academy of Sciences of the United States of America · 2020Article
- The Role of Secretion Systems, Effectors, and Secondary Metabolites of Beneficial Rhizobacteria in Interactions With Plants and Microbes.Frontiers in plant science · 2020Review
- The genome of Ensifer alkalisoli YIC4027 provides insights for host specificity and environmental adaptations.BMC genomics · 2019Article
- Transcriptomic analysis of Rhizobium leguminosarum bacteroids in determinate and indeterminate nodules.Microbial genomics · 2019Article
- Rhizobia: from saprophytes to endosymbionts.Nature reviews. Microbiology · 2018Review
- Two Rieske Fe/S Proteins and TAT System inFrontiers in plant science · 2018Article
- Complete genome of RhizobiumStandards in genomic sciences · 2018Article
- Role of O2 in the Growth of Rhizobium leguminosarum bv. viciae 3841 on Glucose and Succinate.Journal of bacteriology · 2017Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundProteins secreted by bacteria play an important role in infection of eukaryotic hosts. Rhizobia infect the roots of leguminous plants and establish a mutually beneficial symbiosis. Proteins secreted during the infection process by some rhizobial strains can influence infection and modify the plant defence signalling pathways. The aim of this study was to systematically analyse protein secretion in the recently sequenced strain Rhizobium leguminosarum bv. viciae 3841.
resultsSimilarity searches using defined protein secretion systems from other Gram-negative bacteria as query sequences revealed that R. l. bv. viciae 3841 has ten putative protein secretion systems. These are the general export pathway (GEP), a twin-arginine translocase (TAT) secretion system, four separate Type I systems, one putative Type IV system and three Type V autotransporters. Mutations in genes encoding each of these (except the GEP) were generated, but only mutations affecting the PrsDE (Type I) and TAT systems were observed to affect the growth phenotype and the profile of proteins in the culture supernatant. Bioinformatic analysis and mass fingerprinting of tryptic fragments of culture supernatant proteins identified 14 putative Type I substrates, 12 of which are secreted via the PrsDE, secretion system. The TAT mutant was defective for the symbiosis, forming nodules incapable of nitrogen fixation.
conclusionNone of the R. l. bv. viciae 3841 protein secretion systems putatively involved in the secretion of proteins to the extracellular space (Type I, Type IV, Type V) is required for establishing the symbiosis with legumes. The PrsDE (Type I) system was shown to be the major route of protein secretion in non-symbiotic cells and to secrete proteins of widely varied size and predicted function. This is in contrast to many Type I systems from other bacteria, which typically secrete specific substrates encoded by genes often localised in close proximity to the genes encoding the secretion system itself.
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