ArticleProtein science : a publication of the Protein Society2008
Chemical shift assignment of the transmembrane helices of DsbB, a 20-kDa integral membrane enzyme, by 3D magic-angle spinning NMR spectroscopy.
Article in Protein science : a publication of the Protein Society, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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41 citing papers in PubMed, 91 citations in OpenAlex.
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- Lipid bilayer-bound conformation of an integral membrane beta barrel protein by multidimensional MAS NMR.Journal of biomolecular NMR · 2015Article
- On the problem of resonance assignments in solid state NMR of uniformly ¹⁵N,¹³C-labeled proteins.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2015Article
- NMR structures of membrane proteins in phospholipid bilayers.Quarterly reviews of biophysics · 2014Review
- Secondary structure in the core of amyloid fibrils formed from human β₂m and its truncated variant ΔN6.Journal of the American Chemical Society · 2014Article
- Efficient and stable reconstitution of the ABC transporter BmrA for solid-state NMR studies.Frontiers in molecular biosciences · 2014Article
- Resonance assignment of the NMR spectra of disordered proteins using a multi-objective non-dominated sorting genetic algorithm.Journal of biomolecular NMR · 2013Article
- Helical membrane protein conformations and their environment.European biophysics journal : EBJ · 2013Review
- Structure of the disulfide bond generating membrane protein DsbB in the lipid bilayer.Journal of molecular biology · 2013Article
- Efficient resonance assignment of proteins in MAS NMR by simultaneous intra- and inter-residue 3D correlation spectroscopy.Journal of biomolecular NMR · 2013Article
- Yeast-expressed human membrane protein aquaporin-1 yields excellent resolution of solid-state MAS NMR spectra.Journal of biomolecular NMR · 2013Article
- Continuously Tunable 250 GHz Gyrotron with a Double Disk Window for DNP-NMR Spectroscopy.Journal of infrared, millimeter and terahertz waves · 2013Article
- Solid-state NMR analysis of membrane proteins and protein aggregates by proton detected spectroscopy.Journal of biomolecular NMR · 2012Article
- Mutant protein A30P α-synuclein adopts wild-type fibril structure, despite slower fibrillation kinetics.The Journal of biological chemistry · 2012Article
- Structure determination of a membrane protein in proteoliposomes.Journal of the American Chemical Society · 2012Article
- VITAL NMR: using chemical shift derived secondary structure information for a limited set of amino acids to assess homology model accuracy.Journal of biomolecular NMR · 2012Article
- High-resolution membrane protein structure by joint calculations with solid-state NMR and X-ray experimental data.Journal of biomolecular NMR · 2011Article
- Site-specific solid-state NMR detection of hydrogen-deuterium exchange reveals conformational changes in a 7-helical transmembrane protein.Biophysical journal · 2011Article
- Solid-State NMR of a Large Membrane Protein by Paramagnetic Relaxation Enhancement.The journal of physical chemistry letters · 2011Article
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4 authors at 1 institution in 1 country.
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Abstract
The Escherichia coli inner membrane enzyme DsbB catalyzes disulfide bond formation in periplasmic proteins, by transferring electrons to ubiquinone from DsbA, which in turn directly oxidizes cysteines in substrate proteins. We have previously shown that DsbB can be prepared in a state that gives highly resolved magic-angle spinning (MAS) NMR spectra. Here we report sequential 13C and 15N chemical shift assignments for the majority of the residues in the transmembrane helices, achieved by three-dimensional (3D) correlation experiments on a uniformly 13C, 15N-labeled sample at 750-MHz 1H frequency. We also present a four-dimensional (4D) correlation spectrum, which confirms assignments in some highly congested regions of the 3D spectra. Overall, our results show the potential to assign larger membrane proteins using 3D and 4D correlation experiments and form the basis of further structural and dynamical studies of DsbB by MAS NMR.
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