ArticleThe Journal of cell biology2008
Dimeric heat shock protein 40 binds radial spokes for generating coupled power strokes and recovery strokes of 9 + 2 flagella.
Article in The Journal of cell biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 54 citations in OpenAlex.
- Review
- Regulation of p53 and Cancer Signaling by Heat Shock Protein 40/J-Domain Protein Family Members.International journal of molecular sciences · 2021Review
- Rare Human Diseases: Model Organisms in Deciphering the Molecular Basis of Primary Ciliary Dyskinesia.Cells · 2019Review
- The roles of a flagellar HSP40 ensuring rhythmic beating.Molecular biology of the cell · 2019Article
- General and specific promotion of flagellar assembly by a flagellar nucleoside diphosphate kinase.Molecular biology of the cell · 2017Article
- Radial Spokes-A Snapshot of the Motility Regulation, Assembly, and Evolution of Cilia and Flagella.Cold Spring Harbor perspectives in biology · 2017Review
- Mutations in DNAJB13, Encoding an HSP40 Family Member, Cause Primary Ciliary Dyskinesia and Male Infertility.American journal of human genetics · 2016Article
- J domain independent functions of J proteins.Cell stress & chaperones · 2016Review
- Single-particle imaging reveals intraflagellar transport-independent transport and accumulation of EB1 in Chlamydomonas flagella.Molecular biology of the cell · 2016Article
- FAP206 is a microtubule-docking adapter for ciliary radial spoke 2 and dynein c.Molecular biology of the cell · 2015Article
- DNAJB13, a type II HSP40 family member, localizes to the spermatids and spermatozoa during mouse spermatogenesis.BMC developmental biology · 2014Article
- A flagellar A-kinase anchoring protein with two amphipathic helices forms a structural scaffold in the radial spoke complex.The Journal of cell biology · 2012Article
- The DPY-30 domain and its flanking sequence mediate the assembly and modulation of flagellar radial spoke complexes.Molecular and cellular biology · 2012Article
- The versatile molecular complex component LC8 promotes several distinct steps of flagellar assembly.The Journal of cell biology · 2012Article
- Structure and mechanistic insights into novel iron-mediated moonlighting functions of human J-protein cochaperone, Dph4.The Journal of biological chemistry · 2012Article
- Cryoelectron tomography of radial spokes in cilia and flagella.The Journal of cell biology · 2011Article
- Subunit interactions within the Chlamydomonas flagellar spokehead.Cytoskeleton (Hoboken, N.J.) · 2011Article
- The HSP70 chaperone machinery: J proteins as drivers of functional specificity.Nature reviews. Molecular cell biology · 2010Review
- CCTalpha and CCTdelta chaperonin subunits are essential and required for cilia assembly and maintenance in Tetrahymena.PloS one · 2010Article
- Chlamydomonas mutants display reversible deficiencies in flagellar beating and axonemal assembly.Cytoskeleton (Hoboken, N.J.) · 2010Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
T-shape radial spokes regulate flagellar beating. However, the precise function and molecular mechanism of these spokes remain unclear. Interestingly, Chlamydomonas reinhardtii flagella lacking a dimeric heat shock protein (HSP) 40 at the spokehead-spokestalk juncture appear normal in length and composition but twitch actively while cells jiggle without procession, resembling a central pair (CP) mutant. HSP40(-) cells begin swimming upon electroporation with recombinant HSP40. Surprisingly, the rescue doesn't require the signature DnaJ domain. Furthermore, the His-Pro-Asp tripeptide that is essential for stimulating HSP70 adenosine triphosphatase diverges in candidate orthologues, including human DnaJB13. Video microscopy reveals hesitance in bend initiation and propagation as well as irregular stalling and stroke switching despite fairly normal waveform. The in vivo evidence suggests that the evolutionarily conserved HSP40 specifically transforms multiple spoke proteins into stable conformation capable of mechanically coupling the CP with dynein motors. This enables 9 + 2 cilia and flagella to bend and switch to generate alternate power strokes and recovery strokes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.