ArticleMolecular and cellular biology2008
Trypanosoma brucei RNA editing: coupled cycles of U deletion reveal processive activity of the editing complex.
Article in Molecular and cellular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- RNA editing catalytic complexes edit multiple mRNA sites non-processively in Trypanosoma brucei.Molecular and biochemical parasitology · 2023Article
- Site-specific and substrate-specific control of accurate mRNA editing by a helicase complex in trypanosomes.RNA (New York, N.Y.) · 2020Article
- A specific, promoter-independent activity of T7 RNA polymerase suggests a general model for DNA/RNA editing in single subunit RNA Polymerases.Scientific reports · 2018Article
- ARSDA: A New Approach for Storing, Transmitting and Analyzing Transcriptomic Data.G3 (Bethesda, Md.) · 2017Article
- Trypanosome RNA Editing Mediator Complex proteins have distinct functions in gRNA utilization.Nucleic acids research · 2017Article
- In vivo cleavage specificity of Trypanosoma brucei editosome endonucleases.Nucleic acids research · 2017Article
- High-throughput sequencing of partially edited trypanosome mRNAs reveals barriers to editing progression and evidence for alternative editing.RNA (New York, N.Y.) · 2016Article
- Native Variants of the MRB1 Complex Exhibit Specialized Functions in Kinetoplastid RNA Editing.PloS one · 2015Article
- Native mitochondrial RNA-binding complexes in kinetoplastid RNA editing differ in guide RNA composition.RNA (New York, N.Y.) · 2014Article
- Editosome accessory factors KREPB9 and KREPB10 in Trypanosoma brucei.Eukaryotic cell · 2012Article
- When you can't trust the DNA: RNA editing changes transcript sequences.Cellular and molecular life sciences : CMLS · 2011Review
- RNA processing in plant mitochondria is independent of transcription.Plant molecular biology · 2009Article
- Uridine insertion/deletion RNA editing in trypanosomatid mitochondria: In search of the editosome.RNA (New York, N.Y.) · 2009Article
- TbMP42 is a structure-sensitive ribonuclease that likely follows a metal ion catalysis mechanism.Nucleic acids research · 2008Article
- Trypanosome RNA editing: the complexity of getting U in and taking U out.Wiley interdisciplinary reviews. RNAReview
- Uridine insertion/deletion editing in trypanosomes: a playground for RNA-guided information transfer.Wiley interdisciplinary reviews. RNAReview
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Authors and funding
4 authors.
Funding
Abstract
RNA editing in Trypanosoma brucei is posttranscriptional uridylate removal/addition, generally at vast numbers of pre-mRNA sites, but to date, only single editing cycles have been examined in vitro. We here demonstrate achieving sequential cycles of U deletion in vitro, with editing products confirmed by sequence analysis. Notably, the subsequent editing cycle is much more efficient and occurs far more rapidly than single editing cycles; plus, it has different recognition requirements. This indicates that the editing complex acts in a concerted manner and does not dissociate from the RNA substrate between these cycles. Furthermore, the multicycle substrate exhibits editing that is unexpected from a strictly 3'-to-5' progression, reminiscent of the unexpected editing that has been shown to occur frequently in T. brucei mRNAs edited in vivo. This unexpected editing is most likely due to alternate mRNA:guide RNA (gRNA) alignment forming a hyphenated anchor; its having only a 2-bp proximal duplex helps explain the prevalence of unexpected editing in vivo. Such unexpected editing was not previously reported in vitro, presumably because the common use of artificially tight mRNA:gRNA base pairing precludes alternate alignments. The multicycle editing and unexpected editing presented in this paper bring in vitro reactions closer to reproducing the in vivo editing process.
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