ArticleGenome biology2008
A BAC-based integrated linkage map of the silkworm Bombyx mori.
Article in Genome biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 111 citations in OpenAlex.
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- Comparative genomics reveals the dynamics of chromosome evolution in Lepidoptera.Nature ecology & evolution · 2024Article
- Genome Assembly and Population Sequencing Reveal Three Populations and Signatures of Insecticide Resistance of Tuta absoluta in Latin America.Genome biology and evolution · 2023Article
- Exploring the Mystery of the Tetrahydrobiopterin Synthetic Defect Lethal MutantInternational journal of molecular sciences · 2022Article
- SilkBase: an integrated transcriptomic and genomic database for Bombyx mori and related species.Database : the journal of biological databases and curation · 2022Article
- Support for faster and more adaptive Z chromosome evolution in two divergent lepidopteran lineagesEvolution; international journal of organic evolution · 2022Article
- Reference Transcriptome Data in SilkwormInsects · 2021Article
- An update of KAIKObase, the silkworm genome database.Database : the journal of biological databases and curation · 2021Article
- Imaginal disc growth factor maintains cuticle structure and controls melanization in the spot pattern formation of Bombyx mori.PLoS genetics · 2020Article
- Semaphorin-1a-like gene plays an important role in the embryonic development of silkworm, Bombyx mori.PloS one · 2020Article
- Insights into the Structure of the Spruce Budworm (G3 (Bethesda, Md.) · 2018Article
- A single amino acid substitution in the Bombyx-specific mucin-like membrane protein causes resistance to Bombyx mori densovirus.Scientific reports · 2018Article
- Article
- Toll ligand Spätzle3 controls melanization in the stripe pattern formation in caterpillars.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Positional cloning of a Bombyx pink-eyed white egg locus reveals the major role of cardinal in ommochrome synthesis.Heredity · 2016Article
- Mapping and recombination analysis of two moth colour mutations, Black moth and Wild wing spot, in the silkworm Bombyx mori.Heredity · 2016Article
- A comprehensive analysis of the chorion locus in silkmoth.Scientific reports · 2015Article
- Homeodomain Protein Scr Regulates the Transcription of Genes Involved in Juvenile Hormone Biosynthesis in the Silkworm.International journal of molecular sciences · 2015Article
- Functional Loss of Bmsei Causes Thermosensitive Epilepsy in Contractile Mutant Silkworm, Bombyx mori.Scientific reports · 2015Article
Corrections and comments
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Authors and funding
14 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn 2004, draft sequences of the model lepidopteran Bombyx mori were reported using whole-genome shotgun sequencing. Because of relatively shallow genome coverage, the silkworm genome remains fragmented, hampering annotation and comparative genome studies. For a more complete genome analysis, we developed extended scaffolds combining physical maps with improved genetic maps.
resultsWe mapped 1,755 single nucleotide polymorphism (SNP) markers from bacterial artificial chromosome (BAC) end sequences onto 28 linkage groups using a recombining male backcross population, yielding an average inter-SNP distance of 0.81 cM (about 270 kilobases). We constructed 6,221 contigs by fingerprinting clones from three BAC libraries digested with different restriction enzymes, and assigned a total of 724 single copy genes to them by BLAST (basic local alignment search tool) search of the BAC end sequences and high-density BAC filter hybridization using expressed sequence tags as probes. We assigned 964 additional expressed sequence tags to linkage groups by restriction fragment length polymorphism analysis of a nonrecombining female backcross population. Altogether, 361.1 megabases of BAC contigs and singletons were integrated with a map containing 1,688 independent genes. A test of synteny using Oxford grid analysis with more than 500 silkworm genes revealed six versus 20 silkworm linkage groups containing eight or more orthologs of Apis versus Tribolium, respectively.
conclusionThe integrated map contains approximately 10% of predicted silkworm genes and has an estimated 76% genome coverage by BACs. This provides a new resource for improved assembly of whole-genome shotgun data, gene annotation and positional cloning, and will serve as a platform for comparative genomics and gene discovery in Lepidoptera and other insects.
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