ArticlePlant physiology2008
A plasma membrane-anchored fluorescent protein fusion illuminates sieve element plasma membranes in Arabidopsis and tobacco.
Article in Plant physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 67 citations in OpenAlex.
- Modeling the Hydraulic Conductivity of Phloem Sieve Elements.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Immunohistochemical Localization of Proteins in the Phloem: Problems and Solutions.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Live-Cell Imaging of Fluorescently-Tagged Phloem Proteins with Confocal Microscopy.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Split-YFP-coupled interaction-dependent TurboID identifies new functions of basal cell polarity inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Control of plasma membrane-associated actin polymerization specifies the pattern of the cell wall in xylem vessels.Nature communications · 2025Article
- A bifunctional selectable marker for wheat transformation contributes to the characterization of male-sterile phenotype induced by a synthetic Ms2 gene.Plant cell reports · 2023Article
- Coordination Between Phloem Loading and Structure Maintains Carbon Transport Under Drought.Frontiers in plant science · 2022Article
- Seasonal changes in temperate woody plant phloem anatomy and physiology: implications for long-distance transport.AoB PLANTS · 2021Review
- Quantitative Structural Organization of Bulk Apical Membrane Traffic in Pollen Tubes.Plant physiology · 2020Article
- Article
- Simultaneous Acquisition of Multicolor Information From Neural Circuits in Resin-Embedded Samples.Frontiers in neuroscience · 2018Article
- Phloem-Specific Methionine Recycling Fuels Polyamine Biosynthesis in a Sulfur-Dependent Manner and Promotes Flower and Seed Development.Plant physiology · 2016Article
- ClearSee: a rapid optical clearing reagent for whole-plant fluorescence imaging.Development (Cambridge, England) · 2015Article
- Identification of phloem-mobile mRNA.Journal of plant research · 2015Review
- Correlative imaging of fluorescent proteins in resin-embedded plant material.Plant physiology · 2013Article
- P-proteins in Arabidopsis are heteromeric structures involved in rapid sieve tube sealing.Frontiers in plant science · 2013Article
- Sieve element occlusion (SEO) genes encode structural phloem proteins involved in wound sealing of the phloem.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Arabidopsis SUC1 loads the phloem in suc2 mutants when expressed from the SUC2 promoter.Journal of experimental botany · 2012Article
- New insight into silica deposition in horsetail (Equisetum arvense).BMC plant biology · 2011Article
- Callose synthase GSL7 is necessary for normal phloem transport and inflorescence growth in Arabidopsis.Plant physiology · 2011Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rapid acquisition of quantitative anatomical data from the sieve tubes of angiosperm phloem has been confounded by their small size, their distance from organ surfaces, and the time-consuming nature of traditional methods, such as transmission electron microscopy. To improve access to these cells, for which good anatomical data are critical, a monomeric yellow fluorescent protein (mCitrine) was N-terminally fused to a small (approximately 6 kD) membrane protein (AtRCI2A) and stably expressed in Arabidopsis thaliana (Columbia-0 ecotype) and Nicotiana tabacum ('Samsun') under the control of a companion cell-specific promoter (AtSUC2p). The construct, called by its abbreviation SUmCR, yielded stable sieve element (SE) plasma membrane fluorescence labeling, even after plastic (methacrylate) embedding. In conjunction with wide-field fluorescence measurements of sieve pore number and position using aniline blue-stained callose, mCitrine-labeled material was used to calculate rough estimates of sieve tube-specific conductivity for both species. The SUmCR construct also revealed a hitherto unknown expression domain of the AtSUC2 Suc-H(+) symporter in the epidermis of the cell division zone of developing root tips. The success of this construct in targeting plasma membrane-anchored fluorescent proteins to SEs could be attributable to the small size of AtRCI2A or to the presence of other signals innate to AtRCI2A that permit the protein to be trafficked to SEs. The construct provides a hitherto unique entrée into companion cell-to-SE protein targeting, as well as a new tool for studying whole-plant phloem anatomy and architecture.
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