ArticleApplied and environmental microbiology2008
Novel bacterial artificial chromosome vector pUvBBAC for use in studies of the functional genomics of Listeria spp.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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Who cites it
7 citing papers in PubMed, 18 citations in OpenAlex.
- Improved cultivation and metagenomics as new tools for bioprospecting in cold environments.Extremophiles : life under extreme conditions · 2015Review
- Heterologous viral expression systems in fosmid vectors increase the functional analysis potential of metagenomic libraries.Scientific reports · 2013Article
- Protective immunity to Listeria monocytogenes infection mediated by recombinant Listeria innocua harboring the VGC locus.PloS one · 2012Article
- Size Does Matter: Application-driven Approaches for Soil Metagenomics.Soil biology & biochemistry · 2010Article
- Galleria mellonella as a model system for studying Listeria pathogenesis.Applied and environmental microbiology · 2010Article
- Biotechnological applications of Listeria's sophisticated infection strategies.Microbial biotechnology · 2008Review
- Tools for functional postgenomic analysis of listeria monocytogenes.Applied and environmental microbiology · 2008Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial artificial chromosome (BAC) vectors are important tools for microbial genome research. We constructed a novel BAC vector, pUvBBAC, for replication in both gram-negative and gram-positive bacterial hosts. The pUvBBAC vector was used to generate a BAC library for the facultative intracellular pathogen Listeria monocytogenes EGD-e. The library had insert sizes ranging from 68 to 178 kb. We identified two recombinant BACs from the L. monocytogenes pUvBBAC library that each contained the entire virulence gene cluster (vgc) of L. monocytogenes and transferred them to a nonpathogenic Listeria innocua strain. Recombinant L. innocua strains harboring pUvBBAC+vgc1 and pUvBBAC+vgc2 produced the vgc-specific listeriolysin (LLO) and actin assembly protein ActA and represent the first reported cloning of the vgc locus in its entirety. The use of the novel broad-host-range BAC vector pUvBBAC extends the versatility of this technology and provides a powerful platform for detailed functional genomics of gram-positive bacteria as well as its use in explorative functional metagenomics.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.