ArticleJournal of bacteriology2008
Comparative genomics and an insect model rapidly identify novel virulence genes of Burkholderia mallei.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 66 citations in OpenAlex.
- Marmosets as models of infectious diseases.Frontiers in cellular and infection microbiology · 2024Review
- Galleria mellonella-intracellular bacteria pathogen infection models: the ins and outs.FEMS microbiology reviews · 2023Review
- In silico analyses of penicillin binding proteins in Burkholderia pseudomallei uncovers SNPs with utility for phylogeography, species differentiation, and sequence typing.PLoS neglected tropical diseases · 2022Article
- Establishing an invertebrateVirulence · 2021Article
- Galleria mellonella larvae exhibit a weight-dependent lethal median dose when infected with methicillin-resistant Staphylococcus aureus.Pathogens and disease · 2021Article
- Identification ofFrontiers in microbiology · 2020Article
- Recent Advances in the Use ofJournal of fungi (Basel, Switzerland) · 2018Review
- Differences in Virulence BetweenFrontiers in cellular and infection microbiology · 2018Article
- The Madagascar Hissing Cockroach as an Alternative Non-mammalian Animal Model to Investigate Virulence, Pathogenesis, and Drug Efficacy.Journal of visualized experiments : JoVE · 2017Article
- Antibodies againstInfection and immunity · 2017Article
- In vivo efficacy of trans-cinnamaldehyde, carvacrol, and thymol in attenuating Listeria monocytogenes infection in a Galleria mellonella model.Journal of natural medicines · 2016Article
- Galleria mellonella infection models for the study of bacterial diseases and for antimicrobial drug testing.Virulence · 2016Review
- Phenotypic Characterization of a Novel Virulence-Factor Deletion Strain of Burkholderia mallei That Provides Partial Protection against Inhalational Glanders in Mice.Frontiers in cellular and infection microbiology · 2016Article
- Characterization of the Burkholderia mallei tonB Mutant and Its Potential as a Backbone Strain for Vaccine Development.PLoS neglected tropical diseases · 2015Article
- Cross-species comparison of the Burkholderia pseudomallei, Burkholderia thailandensis, and Burkholderia mallei quorum-sensing regulons.Journal of bacteriology · 2014Article
- Use of larvae of the wax moth Galleria mellonella as an in vivo model to study the virulence of Helicobacter pylori.BMC microbiology · 2014Article
- Global analysis of the Burkholderia thailandensis quorum sensing-controlled regulon.Journal of bacteriology · 2014Article
- Mining locus tags in PubMed Central to improve microbial gene annotation.BMC bioinformatics · 2014Article
- Redefining the PF06864 Pfam family based on Burkholderia pseudomallei PilO2(Bp) S-SAD crystal structure.PloS one · 2014Article
- The twin arginine translocation system is essential for aerobic growth and full virulence of Burkholderia thailandensis.Journal of bacteriology · 2014Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Burkholderia pseudomallei and its host-adapted deletion clone Burkholderia mallei cause the potentially fatal human diseases melioidosis and glanders, respectively. The antibiotic resistance profile and ability to infect via aerosol of these organisms and the absence of protective vaccines have led to their classification as major biothreats and select agents. Although documented infections by these bacteria date back over 100 years, relatively little is known about their virulence and pathogenicity mechanisms. We used in silico genomic subtraction to generate their virulome, a set of 650 putative virulence-related genes shared by B. pseudomallei and B. mallei but not present in five closely related nonpathogenic Burkholderia species. Although most of these genes are clustered in putative operons, the number of targets for mutant construction and verification of reduced virulence in animal models is formidable. Therefore, Galleria mellonella (wax moth) larvae were evaluated as a surrogate host; we found that B. pseudomallei and B. mallei, but not other phylogenetically related bacteria, were highly pathogenic for this insect. More importantly, four previously characterized B. mallei mutants with reduced virulence in hamsters or mice had similarly reduced virulence in G. mellonella larvae. Site-specific inactivation of selected genes in the computationally derived virulome identified three new potential virulence genes, each of which was required for rapid and efficient killing of larvae. Thus, this approach may provide a means to quickly identify high-probability virulence genes in B. pseudomallei, B. mallei, and other pathogens.
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