ArticleThe Plant cell2008
EST analysis of hop glandular trichomes identifies an O-methyltransferase that catalyzes the biosynthesis of xanthohumol.
Article in The Plant cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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64 citing papers in PubMed, 195 citations in OpenAlex.
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- FlavonoidInternational journal of molecular sciences · 2026Article
- Association mapping for hop cone chemistry and morphology identifies natural beneficial allele stacks.The plant genome · 2026Article
- A flavin-dependent monooxygenase favors the formation of (6S)-α-bitter acids in hop glandular trichomes.Plant communications · 2026Article
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- A chromosome-scale and haplotype-resolved genome assembly of tetraploid blackberry (Horticulture research · 2025Article
- A dihydrochalcone-specific O-methyltransferase from leaf buds of Populus trichocarpa implicated in bud resin formation.Journal of experimental botany · 2025Article
- Evaluation of drying methods and green extraction techniques to enhance the recovery of bioactive compounds from hop leaves: A sustainable approach for the valorisation of agricultural by-products.Ultrasonics sonochemistry · 2025Article
- Impacts of Mechanical Injury on Volatile Emission Rate and Composition in 45 Subtropical Woody Broad-Leaved Storage and Non-Storage Emitters.Plants (Basel, Switzerland) · 2025Article
- Anticholinesterase Activity and Bioactive Compound Profiling of Six Hop (Foods (Basel, Switzerland) · 2024Article
- Updating the Knowledge on the Secretory Machinery of Hops (Plants (Basel, Switzerland) · 2024Article
- De novo biosynthesis of the hops bioactive flavonoid xanthohumol in yeast.Nature communications · 2024Article
- Article
- DiverseHorticulture research · 2023Article
- Meliaceae genomes provide insights into wood development and limonoids biosynthesis.Plant biotechnology journal · 2023Article
- Functional Diversification and Structural Origins of Plant Natural Product Methyltransferases.Molecules (Basel, Switzerland) · 2022Review
- Fruits of their labour: biotransformation reactions of yeasts during brewery fermentation.Applied microbiology and biotechnology · 2022Review
- Molecular Characterisation of FlavanoneInternational journal of molecular sciences · 2022Article
- The Potential of Cold Plasma and Electromagnetic Field as Stimulators of Natural Sweeteners Biosynthesis inPlants (Basel, Switzerland) · 2022Article
- Diversification of Chemical Structures of Methoxylated Flavonoids and Genes Encoding Flavonoid-Plants (Basel, Switzerland) · 2022Review
4 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The glandular trichomes (lupulin glands) of hop (Humulus lupulus) synthesize essential oils and terpenophenolic resins, including the bioactive prenylflavonoid xanthohumol. To dissect the biosynthetic processes occurring in lupulin glands, we sequenced 10,581 ESTs from four trichome-derived cDNA libraries. ESTs representing enzymes of terpenoid biosynthesis, including all of the steps of the methyl 4-erythritol phosphate pathway, were abundant in the EST data set, as were ESTs for the known type III polyketide synthases of bitter acid and xanthohumol biosynthesis. The xanthohumol biosynthetic pathway involves a key O-methylation step. Four S-adenosyl-l-methionine-dependent O-methyltransferases (OMTs) with similarity to known flavonoid-methylating enzymes were present in the EST data set. OMT1, which was the most highly expressed OMT based on EST abundance and RT-PCR analysis, performs the final reaction in xanthohumol biosynthesis by methylating desmethylxanthohumol to form xanthohumol. OMT2 accepted a broad range of substrates, including desmethylxanthohumol, but did not form xanthohumol. Mass spectrometry and proton nuclear magnetic resonance analysis showed it methylated xanthohumol to 4-O-methylxanthohumol, which is not known from hop. OMT3 was inactive with all substrates tested. The lupulin gland-specific EST data set expands the genomic resources for H. lupulus and provides further insight into the metabolic specialization of glandular trichomes.
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