ArticleMolecular microbiology2008
Requirements for translation re-initiation in Escherichia coli: roles of initiator tRNA and initiation factors IF2 and IF3.
Article in Molecular microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- A Microscale Platform for the Comprehensive Analysis of Bacterial Translation Initiation.International journal of molecular sciences · 2026Article
- Leaky recombinant expression reveals design constraints of bicistronic synthetic operons in Escherichia coli.Scientific reports · 2026Article
- Translational coupling of neighboring genes in prokaryotes.Journal of bacteriology · 2025Review
- Kinetics of programmed and spontaneous ribosome sliding along the mRNA.Nucleic acids research · 2024Article
- Bicistronic design as recombinant expression enhancer: characteristics, applications, and structural optimization.Applied microbiology and biotechnology · 2021Review
- Translational coupling via termination-reinitiation in archaea and bacteria.Nature communications · 2019Article
- Evaluation of 244,000 synthetic sequences reveals design principles to optimize translation in Escherichia coli.Nature biotechnology · 2018Article
- Identification of theApplied and environmental microbiology · 2017Article
- An extended Shine-Dalgarno sequence in mRNA functionally bypasses a vital defect in initiator tRNA.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Histidine biosynthesis, its regulation and biotechnological application in Corynebacterium glutamicum.Microbial biotechnology · 2014Review
- Article
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2 authors.
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Abstract
Despite its importance in post-transcriptional regulation of polycistronic operons in Escherichia coli, little is known about the mechanism of translation re-initiation, which occurs when the same ribosome used to translate an upstream open reading frame (ORF) also translates a downstream ORF. To investigate translation re-initiation in Escherichia coli, we constructed a di-cistronic reporter in which a firefly luciferase gene was linked to a chloramphenicol acetyltransferase gene using a segment of the translationally coupled geneV-geneVII intercistronic region from M13 phage. With this reporter and mutant initiator tRNAs, we show that two of the unique properties of E. coli initiator tRNA - formylation of the amino acid attached to the tRNA and binding of the tRNA to the ribosomal P-site - are as important for re-initiation as for de novo initiation. Overexpression of IF2 or increasing the affinity of mutant initiator tRNA for IF2 enhanced re-initiation efficiency, suggesting that IF2 is required for efficient re-initiation. In contrast, overexpression of IF3 led to a marked decrease in re-initiation efficiency, suggesting that a 30S ribosome and not a 70S ribosome is used for translation re-initiation. Strikingly, overexpression of IF3 also blocked E. coli from acting as a host for propagation of M13 phage.
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