ArticleMolecular microbiology2008
Mitochondrial fatty acid synthesis is required for normal mitochondrial morphology and function in Trypanosoma brucei.
Article in Molecular microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 67 citations in OpenAlex.
- A defect in mitochondrial fatty acid synthesis impairs iron metabolism and causes elevated ceramide levels.Nature metabolism · 2023Article
- Fatty acid uptake inFrontiers in cellular and infection microbiology · 2022Review
- Lipid and fatty acid metabolism in trypanosomatids.Microbial cell (Graz, Austria) · 2021Review
- A broadly active fucosyltransferase LmjFUT1 whose mitochondrial localization and activity are essential in parasiticProceedings of the National Academy of Sciences of the United States of America · 2021Article
- The mitochondrial peroxiredoxin displays distinct roles in different developmental stages of African trypanosomes.Redox biology · 2020Article
- Article
- Identifying off-target effects of etomoxir reveals that carnitine palmitoyltransferase I is essential for cancer cell proliferation independent of β-oxidation.PLoS biology · 2018Article
- Molecular model of the mitochondrial genome segregation machinery inProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Gene expression to mitochondrial metabolism: Variability among cultured Trypanosoma cruzi strains.PloS one · 2018Article
- Lipidomics and anti-trypanosomatid chemotherapy.Clinical and translational medicine · 2017Review
- The Glycerol-3-Phosphate Acyltransferase TbGAT is Dispensable for Viability and the Synthesis of Glycerolipids in Trypanosoma brucei.The Journal of eukaryotic microbiology · 2016Article
- Trypanocidal action of bisphosphonium salts through a mitochondrial target in bloodstream form Trypanosoma brucei.International journal for parasitology. Drugs and drug resistance · 2016Article
- A Trypanosomatid Iron Transporter that Regulates Mitochondrial Function Is Required for Leishmania amazonensis Virulence.PLoS pathogens · 2016Article
- Flagellar membranes are rich in raft-forming phospholipids.Biology open · 2015Article
- ATPaseTb2, a unique membrane-bound FoF1-ATPase component, is essential in bloodstream and dyskinetoplastic trypanosomes.PLoS pathogens · 2015Article
- The mitochondrial complex I of trypanosomatids--an overview of current knowledge.Journal of bioenergetics and biomembranes · 2014Review
- Acylation in trypanosomatids: an essential process and potential drug target.Trends in parasitology · 2014Review
- The threonine degradation pathway of the Trypanosoma brucei procyclic form: the main carbon source for lipid biosynthesis is under metabolic control.Molecular microbiology · 2013Article
- Lipid synthesis in protozoan parasites: a comparison between kinetoplastids and apicomplexans.Progress in lipid research · 2013Review
- Mitochondrial shape and function in trypanosomes requires the outer membrane protein, TbLOK1.Molecular microbiology · 2013Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Trypanosoma brucei use microsomal elongases for de novo synthesis of most of its fatty acids. In addition, this parasite utilizes an essential mitochondrial type II synthase for production of octanoate (a lipoic acid precursor) as well as longer fatty acids such as palmitate. Evidence from other organisms suggests that mitochondrially synthesized fatty acids are required for efficient respiration but the exact relationship remains unclear. In procyclic form trypanosomes, we also found that RNAi depletion of the mitochondrial acyl carrier protein, an important component of the fatty acid synthesis machinery, significantly reduces cytochrome-mediated respiration. This reduction was explained by RNAi-mediated inhibition of respiratory complexes II, III and IV, but not complex I. Other effects of RNAi, such as changes in mitochondrial morphology and alterations in membrane potential, raised the possibility of a change in mitochondrial membrane composition. Using mass spectrometry, we observed a decrease in total and mitochondrial phosphatidylinositol and mitochondrial phosphatidylethanolamine. Thus, we conclude that the mitochondrial synthase produces fatty acids needed for maintaining local phospholipid levels that are required for activity of respiratory complexes and preservation of mitochondrial morphology and function.
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