ReviewMolecular biotechnology2008
PCR-SSCP: a method for the molecular analysis of genetic diseases.
Review in Molecular biotechnology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- Association Between Lactoferrin Gene Polymorphism and Dental Caries in Saudi Children: A Case-Control Study.Cureus · 2025Article
- From regulation to deregulation of p53 in hematologic malignancies: implications for diagnosis, prognosis and therapy.Biomarker research · 2024Review
- Meta-analysis of biodynamic (BD) preparations reveal the bacterial population involved in improving soil health, crop yield and quality.Journal, genetic engineering & biotechnology · 2024Article
- Monitoring Drought Tolerance in Oil Palm: Choline Monooxygenase as a Novel Molecular Marker.Plants (Basel, Switzerland) · 2023Article
- Development of allele-specific PCR methodology (AS-PCR) to screening A. lumbricoides and A. suum.Parasitology research · 2022Article
- Glycogen Storage Disease Type Ia Screening Using Dried Blood Spots on Filter Paper: Application of COP-PCR for Detection of the c.648G>T G6PC Gene Mutation.The Kobe journal of medical sciences · 2021Article
- Sensitivity and applications of the PCR Single-Strand Conformation Polymorphism method.Molecular biology reports · 2021Review
- Diagnostics of Mutations in MMR/Diagnostics (Basel, Switzerland) · 2020Review
- Identification of Two Novel Mutations in KDM3A Regulatory Gene in Iranian Infertile MalesIranian biomedical journal · 2019Article
- Molecular diagnosis of glycogen storage disease type I: a review.EXCLI journal · 2019Review
- The profiling of pre- and post-warming DNA in mouse embryos with microsatellite method.Veterinary world · 2018Article
- MALDI-TOF-MS based identification and molecular characterization of food associated methicillin-resistant Staphylococcus aureus.Scientific reports · 2017Article
- Effective screen of CRISPR/Cas9-induced mutants in rice by single-strand conformation polymorphism.Plant cell reports · 2016Article
- Development of low-density oligonucleotide microarrays for detecting mutations causing Wilson's disease.The Indian journal of medical research · 2015Article
- Article
- An overview of mutation detection methods in genetic disorders.Iranian journal of pediatrics · 2013Review
- Genotype analysis of collagenase gene by PCR-SSCP in Vibrio alginolyticus and its association with virulence to marine fish.Current microbiology · 2011Article
- Is single-strand conformation polymorphism analysis of the full 5' untranslated region an adequate approach to study hepatitis C virus quasispecies distribution?Journal of virology · 2009Article
- Separation of 1-23-kb complementary DNA strands by urea-agarose gel electrophoresis.Nucleic acids research · 2009Article
- The Infestation of Supplied Mincemeat toIranian journal of parasitologyArticle
Corrections and comments
- Erratum issuedKonstantinos, Kakavas V [corrected to Kakavas, V Konstantinos]; Panagiotis, Plageras [corrected to Plageras, Panagiotis]; Antonios, Vlachos T [corrected to Vlachos, T Antonios]; Agelos, Papaioannou [corrected to Papaioannou, Agelos]; Argiris, Noulas V [cor
Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single strand conformation polymorphism (SSCP) is a reproducible, rapid and quite simple method for the detection of deletions/insertions/rearrangements in polymerase chain reaction amplified DNA. All the details for the use of PCR-SSCP are presented in the direction of genetic diseases (beta-thalassaemia, cystic fibrosis), optimum gel conditions, sensitivity and the latest modifications of the method, which are applied in most laboratories. This non-radioactive PCR-SSCP method can be reliably used to identify mutations in patients (beta-globin, CFTR), provided suitable controls are available. Moreover, it is widely used for mutation identification in carriers (beta-thalassaemia, cystic fibrosis), making it particularly useful in population screening.
Indexed as
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.