ArticleEndocrinology2008
Progestin-regulated luminal cell and myoepithelial cell-specific responses in mammary organoid culture.
Article in Endocrinology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 29 citations in OpenAlex.
- Progesterone modulates cell growth via integrin αvβ3-dependent pathway in progesterone receptor-negative MDA-MB-231 cells.Heliyon · 2024Article
- A closer look into the cellular and molecular biology of myoepithelial cells across various exocrine glands.The ocular surface · 2024Review
- Progesterone and Breast Cancer.Endocrine reviews · 2020Review
- RANKL/RANK/OPG system beyond bone remodeling: involvement in breast cancer and clinical perspectives.Journal of experimental & clinical cancer research : CR · 2019Review
- C/EBPβ LIP and c-Jun synergize to regulate expression of the murine progesterone receptor.Molecular and cellular endocrinology · 2018Article
- Functional roles of MMP14 and MMP15 in early postnatal mammary gland development.Development (Cambridge, England) · 2016Article
- Wnt and Neuregulin1/ErbB signalling extends 3D culture of hormone responsive mammary organoids.Nature communications · 2016Article
- Luminal STAT5 mediates H2AX promoter activity in distinct population of basal mammary epithelial cells.Oncotarget · 2016Article
- Article
- Epidermal growth factor receptor (EGFR) signaling is a key mediator of hormone-induced leukocyte infiltration in the pubertal female mammary gland.Endocrinology · 2014Article
- Aldehyde dehydrogenase and estrogen receptor define a hierarchy of cellular differentiation in the normal human mammary epithelium.Breast cancer research : BCR · 2014Article
- Progesterone receptor and Stat5 signaling cross talk through RANKL in mammary epithelial cells.Molecular endocrinology (Baltimore, Md.) · 2013Article
- Amphiregulin mediates progesterone-induced mammary ductal development during puberty.Breast cancer research : BCR · 2013Article
- Examining the pathogenesis of breast cancer using a novel agent-based model of mammary ductal epithelium dynamics.PloS one · 2013Article
- A potential role of progestin-induced laminin-5/α6-integrin signaling in the formation of side branches in the mammary gland.Endocrinology · 2012Article
- The biology of progesterone receptor in the normal mammary gland and in breast cancer.Molecular and cellular endocrinology · 2012Review
- MiR-126-3p regulates progesterone receptors and involves development and lactation of mouse mammary gland.Molecular and cellular biochemistry · 2011Article
- Article
- Retaining cell-cell contact enables preparation and culture of spheroids composed of pure primary cancer cells from colorectal cancer.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Estrogen and progesterone in normal mammary gland development and in cancer.Hormones & cancer · 2011Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Normal mammary gland development requires the coordinated proliferation and morphogenesis of both mammary luminal epithelial cells (LECs) and myoepithelial cells (MECs). Cell proliferation in cultured mammary organoids containing both LECs and MECs is not increased by progestin (R5020) or 17beta-estradiol (E2) alone or R5020+E2 but is increased by E2-regulated, mammary stroma-derived Hepatocyte growth factor (HGF) and further increased by HGF+R5020. We investigated the effects of HGF and/or R5020 on morphology and LEC- and MEC-specific in vitro proliferation in organoids. HGF-induced tubulogenesis was initiated and carried out by LECs starting with cellular extensions, followed by the formation of chains and cords, and culminating in tubule formation. MECs did not appear to have an active role in this process. Whereas HGF by itself caused maximal proliferation of LECs, HGF+R5020 produced a synergistic and specific increase in MEC proliferation. Because only LECs expressed progesterone receptors (PRs), we investigated the role of receptor activator of nuclear factor-kappaB ligand (RANKL), a progestin-induced paracrine factor, in mediating increased MEC proliferation. Quantitative RT-PCR showed that RANKL mRNA was induced by R5020 or HGF+R5020 and RANKL protein colocalized with PRs in LECs. The increased proliferation of MECs in response to HGF+R5020 could be blocked by neutralizing antibody to RANKL and reproduced by treatment with HGF plus exogenous RANKL in place of R5020. Neither R5020, nor exogenously administered RANKL increased proliferation of LECs. These results led us to conclude that RANKL, induced by progestin in PR-positive cells, is secreted and interacts with HGF to specifically increase proliferation of PR-negative MECs.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.