ArticleAnalytical chemistry2008
Catch strip assay for the relative assessment of two-dimensional protein association kinetics.
Article in Analytical chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
5 citing papers in PubMed.
- E-selectin-mediated rolling facilitates pancreatic cancer cell adhesion to hyaluronic acid.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2017Article
- P-Selectin and ICAM-1 synergy in mediating THP-1 monocyte adhesion in hemodynamic flow is length dependent.Integrative biology : quantitative biosciences from nano to macro · 2017Article
- Effects of shear on P-selectin deposition in microfluidic channels.Biomicrofluidics · 2016Article
- Nano-motion dynamics are determined by surface-tethered selectin mechanokinetics and bond formation.PLoS computational biology · 2009Article
- Action at a distance: lengthening adhesion bonds with poly(ethylene glycol) spacers enhances mechanically stressed affinity for improved vascular targeting of microparticles.Langmuir : the ACS journal of surfaces and colloids · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Accurate interpretation of recruitment rate measurements of microscale particles, such as cells and microbeads, to biofunctional surfaces is difficult because factors such as uneven ligand distributions, particle collisions, variable particle fluxes, and molecular-scale surface separation distances obfuscate the ability to link the observed particle behavior with the governing nanoscale biophysics. We report the development of a hydrodynamically conditioned micropattern catch strip assay to measure microparticle recruitment kinetics. The assay exploited patterning within microfluidic channels and the mechanostability of selectin bonds to create reaction geometries that confined a microbead flux to within 200 nm of the surface under flow conditions. Systematic control of capillary action enabled the creation of homogeneous or gradient ligand distributions. The method enabled the measurement of particle recruitment rates (keff, s-1) that were primarily determined by the interaction of the biomolecular pair being investigated. The method is therefore well suited for relative measurements of delivery vehicle and cellular recruitment potential as governed by surface-bound molecules.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.