ArticleThe Plant cell1991
Analysis of rice Act1 5' region activity in transgenic rice plants.
Article in The Plant cell, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.
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Who cites it
80 citing papers in PubMed.
- Varying the expression pattern of the strigolactone receptor geneFrontiers in plant science · 2023Article
- Target Lines forInternational journal of molecular sciences · 2022Article
- Transgenic rice plants expressing the α-L-arabinofuranosidase ofPlant biotechnology (Tokyo, Japan) · 2021Article
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- A Fruitful Decade Using Synthetic Promoters in the Improvement of Transgenic Plants.Frontiers in plant science · 2019Review
- A Plant-SpecificFrontiers in plant science · 2019Article
- Characterization of a strong and constitutive promoter from the Arabidopsis serine carboxypeptidase-like gene AtSCPL30 as a potential tool for crop transgenic breeding.BMC biotechnology · 2018Article
- Whitefly and aphid inducible promoters of Arabidopsis thaliana L.Journal of genetics · 2018Article
- The MAP3K-CodingFrontiers in plant science · 2018Article
- Development of gene expression system in egg cells and zygotes isolated from rice and maize.Plant direct · 2017Article
- Comparison of the impact of viral and plant-derived promoters regulating selectable marker gene on maize transformation and transgene expression.Plant cell reports · 2017Article
- A Lin28 homologue reprograms differentiated cells to stem cells in the moss Physcomitrella patens.Nature communications · 2017Article
- Identification and functional characterization of the NAC gene promoter from Populus euphratica.Planta · 2016Article
- A petal-specific InMYB1 promoter from Japanese morning glory: a useful tool for molecular breeding of floricultural crops.Plant biotechnology journal · 2016Article
- Functional architecture of two exclusively late stage pollen-specific promoters in rice (Oryza sativa L.).Plant molecular biology · 2015Article
- Article
- Overexpression of a Gene Involved in Phytic Acid Biosynthesis Substantially Increases Phytic Acid and Total Phosphorus in Rice Seeds.Plants (Basel, Switzerland) · 2015Article
- Upstream regulatory architecture of rice genes: summarizing the baseline towards genus-wide comparative analysis of regulatory networks and allele mining.Rice (New York, N.Y.) · 2015Article
- Isolation and functional characterization of a novel rice constitutive promoter.Plant cell reports · 2014Article
- Progress of cereal transformation technology mediated by Agrobacterium tumefaciens.Frontiers in plant science · 2014Review
20 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors.
Funding
Abstract
The 5' region of the rice actin 1 gene (Act1) has been developed as an efficient regulator of foreign gene expression in transgenic rice plants. To determine the pattern and level of rice Act1 5' region activity, transgenic rice plants containing the Act1 5' region fused to a bacterial beta-glucuronidase (Gus) coding sequence were generated. Two independent clonal lines of transgenic rice plants were analyzed in detail. Quantitative analysis showed that tissue from these transgenic rice plants have a level of GUS protein that represents as much as 3% of total soluble protein. We were able to demonstrate that Act1-Gus gene expression is constitutive throughout the sporophytic and gametophytic tissues of these transgenic rice plants. Plants from one transgenic line were analyzed for the segregation of GUS activity in pollen by in situ histochemical staining, and the inheritance and stability of Act1-Gus expression were assayed in subsequently derived progeny plants.
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