ArticleAmerican journal of physiology. Renal physiology2008
Expression and function of rat urothelial P2Y receptors.
Article in American journal of physiology. Renal physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 67 citations in OpenAlex.
- Biomechanical regulation of bladder injury and repair.Nature reviews. Urology · 2026Review
- Purinergic Receptor P2Y2 Regulates Neurogenesis and Functional Recovery Following Spinal Cord Injury in Zebrafish.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Article
- Mechanosensitive release of ATP in the urinary bladder mucosa.Purinergic signalling · 2025Review
- Review
- Time-dependent photobiomodulation management of neuropathic pain induced by spinal cord injury in male rats.Lasers in medical science · 2023Article
- Transient receptor potential vanilloid type 4 (TRPV4) in urinary bladder structure and function.Current topics in membranes · 2022Review
- Sophisticated regulation of micturition: review of basic neurourology.Journal of exercise rehabilitation · 2021Review
- The Urothelium: Life in a Liquid Environment.Physiological reviews · 2020Review
- TRPV4 receptor as a functional sensory molecule in bladder urothelium: Stretch-independent, tissue-specific actions and pathological implications.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Purinergic receptor mediated calcium signalling in urothelial cells.Scientific reports · 2019Article
- Purinergic P2X7 receptors as therapeutic targets in interstitial cystitis/bladder pain syndrome; key role of ATP signaling in inflammation.Bladder (San Francisco, Calif.) · 2019Review
- Urothelial purine release during filling of murine and primate bladders.American journal of physiology. Renal physiology · 2016Article
- Purinergic signalling underlies transforming growth factor-β-mediated bladder afferent nerve hyperexcitability.The Journal of physiology · 2016Article
- Receptors, channels, and signalling in the urothelial sensory system in the bladder.Nature reviews. Urology · 2016Review
- Pannexin 1 channels mediate the release of ATP into the lumen of the rat urinary bladder.The Journal of physiology · 2015Article
- Neural control of the lower urinary tract.Comprehensive Physiology · 2015Review
- Activation of P2Y6 receptors increases the voiding frequency in anaesthetized rats by releasing ATP from the bladder urothelium.British journal of pharmacology · 2014Article
- Bladder sensory physiology: neuroactive compounds and receptors, sensory transducers, and target-derived growth factors as targets to improve function.American journal of physiology. Regulatory, integrative and comparative physiology · 2014Review
- Purinergic signalling in the urinary tract in health and disease.Purinergic signalling · 2014Review
- Purinergic and muscarinic modulation of ATP release from the urothelium and its paracrine actions.American journal of physiology. Renal physiology · 2014Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The control and regulation of the lower urinary tract are partly mediated by purinergic signaling. This study investigated the distribution and function of P2Y receptors in the rat urinary bladder. Application of P2Y agonists to rat urothelial cells evoked increases in intracellular calcium; the rank order of agonist potency (pEC(50) +/- SE) was ATP (5.10 +/- 0.07) > UTP (4.91 +/- 0.14) > UTPgammaS (4.61 +/- 0.16) = ATPgammaS (4.70 +/- 0.05) > 2-methylthio adenosine 5'-diphosphate = 5'-(N-ethylcarboxamido)adenosine = ADP (<3.5). The rank order potency for these agonists indicates that urothelial cells functionally express P2Y(2)/P2Y(4) receptors, with a relative lack of contribution from other P2Y or adenosine receptors. Real-time PCR, Western blotting, and immunocytochemistry confirmed the expression of P2Y(2) and to a lesser extent P2Y(4) in the urothelium. Immunocytochemical studies revealed expression of P2Y(2) staining in all layers of the urothelium, with relative absence of P2Y(4). P2Y(2) staining was also present in suburothelial nerve bundles and underlying detrusor smooth muscle. Addition of UTP and UTPgammaS was found to evoke ATP release from cultured rat urothelial cells. These findings indicate that cultured rat urothelial cells functionally express P2Y(2)/P2Y(4) receptors. Activation of these receptors could have a role in autocrine and paracrine signaling throughout the urothelium. This could lead to the release of bioactive mediators such as additional ATP, nitric oxide, and acetylcholine, which can modulate the micturition reflex by acting on suburothelial myofibroblasts and/or pelvic afferent fibers.
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