ArticleChemistry & biology2008
Analysis of the substrate specificity of the Dim-5 histone lysine methyltransferase using peptide arrays.
Article in Chemistry & biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 72 citations in OpenAlex.
- Machine learning-driven prediction of substrates for enzymes introducing or removing protein post-translational modifications.Communications chemistry · 2025Article
- Uncovering Enzyme-Specific Post-Translational Modifications: An Overview of Current Methods.Proteomes · 2025Review
- H3T11 phosphorylation by CKII is required for heterochromatin formation in Neurospora.Nucleic acids research · 2024Article
- Evaluation of Jumonji C lysine demethylase substrate preference to guide identification ofSTAR protocols · 2022Article
- Insights into a Cancer-Target Demethylase: Substrate Prediction through Systematic Specificity Analysis for KDM3A.Biomolecules · 2022Article
- Specificity Analysis of Protein Methyltransferases and Discovery of Novel Substrates Using SPOT Peptide Arrays.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Structure, Activity and Function of the Suv39h1 and Suv39h2 Protein Lysine Methyltransferases.Life (Basel, Switzerland) · 2021Review
- An engineered variant of SETD3 methyltransferase alters target specificity from histidine to lysine methylation.The Journal of biological chemistry · 2020Article
- Using Yeast to Define the Regulatory Role of Protein Lysine Methylation.Current protein & peptide science · 2020Review
- Structural basis for the target specificity of actin histidine methyltransferase SETD3.Nature communications · 2019Article
- Tailoring Proteins to Re-Evolve Nature: A Short Review.Molecular biotechnology · 2018Review
- Metabolic intermediates - Cellular messengers talking to chromatin modifiers.Molecular metabolism · 2018Review
- Article
- The impact of cellular metabolism on chromatin dynamics and epigenetics.Nature cell biology · 2017Review
- Substrate Specificity of the HEMK2 Protein Glutamine Methyltransferase and Identification of Novel Substrates.The Journal of biological chemistry · 2016Article
- Substrate Specificity Profiling of Histone-Modifying Enzymes by Peptide Microarray.Methods in enzymology · 2016Article
- Histones: at the crossroads of peptide and protein chemistry.Chemical reviews · 2015Review
- Somatic cancer mutations in the MLL3-SET domain alter the catalytic properties of the enzyme.Clinical epigenetics · 2015Article
- An unexpected journey: lysine methylation across the proteome.Biochimica et biophysica acta · 2014Review
- Specificity analysis of protein lysine methyltransferases using SPOT peptide arrays.Journal of visualized experiments : JoVE · 2014Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Histone methylation is an epigenetic mark essential for gene regulation and development. We introduce peptide SPOT synthesis to study sequence specificity of the Dim-5 histone-3 lysine-9 methyltransferase. Dim-5 recognizes R8-G12 of the H3 tail with T11 and G12 being the most important specificity determinants. Exchange of H3 tail residue S10 and T11 by E strongly reduced methylation by Dim-5, suggesting that phosphorylation of S10 or T11 may regulate the activity of Dim-5. In the Dim-5/peptide structure, E227 interacts with H3R8 and D209 with H3-S10. Mutations of E227 or D209 caused predictable changes in the substrate preference, illustrating that peptide recognition of histone methyltransferases can be altered by protein design. Comparative analyses of peptide arrays with wild-type and mutant enzymes, therefore, are well suited to investigate the target specificity of protein methyltransferases and study epigenetic crosstalk.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.