ArticleBMC developmental biology2008
Lactation failure in Src knockout mice is due to impaired secretory activation.
Article in BMC developmental biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 48 citations in OpenAlex.
- Genetic Diversity, Population Structure and Selection Signature in Begait Goats Revealed by Whole-Genome Sequencing.Animals : an open access journal from MDPI · 2024Article
- Medicinal MushroomEvidence-based complementary and alternative medicine : eCAM · 2022Article
- Occludin is a target of Src kinase and promotes lipid secretion by binding to BTN1a1 and XOR.PLoS biology · 2022Article
- Phenotypic Characterization and Brain Structure Analysis of Calcium Channel Subunit αFrontiers in synaptic neuroscience · 2021Article
- Inhibition of the Activity of Cyclophilin A Impedes Prolactin Receptor-Mediated Signaling, Mammary Tumorigenesis, and Metastases.iScience · 2020Article
- Rapid Communication: Genome-wide association analyses identify loci associated with colostrum production in Jersey cattle1.Journal of animal science · 2019Article
- Milk lipid regulation at the maternal-offspring interface.Seminars in cell & developmental biology · 2018Review
- Th-POK regulates mammary gland lactation through mTOR-SREBP pathway.PLoS genetics · 2018Article
- Zinc transporter 2 interacts with vacuolar ATPase and is required for polarization, vesicle acidification, and secretion in mammary epithelial cells.The Journal of biological chemistry · 2017Article
- ZnT4 (SLC30A4)-null ("lethal milk") mice have defects in mammary gland secretion and hallmarks of precocious involution during lactation.American journal of physiology. Regulatory, integrative and comparative physiology · 2016Article
- Actin filament-associated protein 1 is required for cSrc activity and secretory activation in the lactating mammary gland.Oncogene · 2015Article
- New insights in prolactin: pathological implications.Nature reviews. Endocrinology · 2015Review
- Inhibition of SRC family kinases protects hippocampal neurons and improves cognitive function after traumatic brain injury.Journal of neurotrauma · 2014Article
- Mouse mammary tumor virus suppresses apoptosis of mammary epithelial cells through ITAM-mediated signaling.Journal of virology · 2012Article
- SnoN regulates mammary gland alveologenesis and onset of lactation by promoting prolactin/Stat5 signaling.Development (Cambridge, England) · 2012Article
- The RhoA-Rok-myosin II pathway is involved in extracellular matrix-mediated regulation of prolactin signaling in mammary epithelial cells.Journal of cellular physiology · 2012Article
- Signal transducer and activator of transcription 5 as a key signaling pathway in normal mammary gland developmental biology and breast cancer.Breast cancer research : BCR · 2011Review
- Article
- Article
- Correlation analysis between three novel SNPs of the Src gene in bovine and milk production traits.Molecular biology reports · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundMammary gland development culminates in lactation and is orchestrated by numerous stimuli and signaling pathways. The Src family of nonreceptor tyrosine kinases plays a pivotal role in cell signaling. In order to determine if Src plays a role in mammary gland development we have examined mammary gland development and function during pregnancy and lactation in mice in which expression of Src has been eliminated.
resultsWe have characterized a lactation defect in the Src-/- mice which results in the death of over 80% of the litters nursed by Src-/- dams. Mammary gland development during pregnancy appears normal in these mice; however secretory activation does not seem to occur. Serum prolactin levels are normal in Src-/- mice compared to wildtype controls. Expression of the prolactin receptor at both the RNA and protein level was decreased in Src-/- mice following the transition from pregnancy to lactation, as was phosphorylation of STAT5 and expression of milk protein genes. These results suggest that secretory activation, which occurs following parturition, does not occur completely in Src-/- mice. Failed secretory activation results in precocious involution in the mammary glands of Src-/- even when pups were suckling. Involution was accelerated following pup withdrawal perhaps as a result of incomplete secretory activation. In vitro differentiation of mammary epithelial cells from Src-/- mice resulted in diminished production of milk proteins compared to the amount of milk proteins produced by Src+/+ cells, indicating a direct role for Src in regulating the transcription/translation of milk protein genes in mammary epithelial cells.
conclusionSrc is an essential signaling modulator in mammary gland development as Src-/- mice exhibit a block in secretory activation that results in lactation failure and precocious involution. Src appears to be required for increased expression of the prolactin receptor and successful downstream signaling, and alveolar cell organization.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.