ArticleBMC microbiology2008
Pleiotropic phenotypes of a Yersinia enterocolitica flhD mutant include reduced lethality in a chicken embryo model.
Article in BMC microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 21 citations in OpenAlex.
- Comparative pangenome analysis of Yersinia enterocolitica in a one health approach.BMC genomics · 2025Article
- Evaluation of the Correlation between the mRNA Expression Levels ofPathogens (Basel, Switzerland) · 2021Article
- The use of embryonic chicken eggs as an alternative model to evaluate the virulence of Salmonella enterica serovar Gallinarum.PloS one · 2020Article
- Isolate Specific Cold Response ofFrontiers in microbiology · 2019Article
- Differential impact of lipopolysaccharide defects caused by loss of RfaH in Yersinia pseudotuberculosis and Yersinia pestis.Scientific reports · 2017Article
- Expanding the use of alternative models to investigate novel aspects of immunity to microbial pathogens.Virulence · 2014Article
- Flagellum density regulates Proteus mirabilis swarmer cell motility in viscous environments.Journal of bacteriology · 2013Article
- Regulation of cell division, biofilm formation, and virulence by FlhC in Escherichia coli O157:H7 grown on meat.Applied and environmental microbiology · 2011Article
- OmpR controls Yersinia enterocolitica motility by positive regulation of flhDC expression.Antonie van Leeuwenhoek · 2011Article
- Embryonated eggs as an alternative infection model to investigate Aspergillus fumigatus virulence.Infection and immunity · 2010Article
- Francisella tularensis DeltapyrF mutants show that replication in nonmacrophages is sufficient for pathogenesis in vivo.Infection and immunity · 2010Article
- Global transcriptional response to mammalian temperature provides new insight into Francisella tularensis pathogenesis.BMC microbiology · 2008Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe Yersinia enterocolitica flagellar master regulator FlhD/FlhC affects the expression levels of non-flagellar genes, including 21 genes that are involved in central metabolism. The sigma factor of the flagellar system, FliA, has a negative effect on the expression levels of seven plasmid-encoded virulence genes in addition to its positive effect on the expression levels of eight of the flagellar operons. This study investigates the phenotypes of flhD and fliA mutants that result from the complex gene regulation.
resultsPhenotypes relating to central metabolism were investigated with Phenotype MicroArrays. Compared to the wild-type strain, isogenic flhD and fliA mutants exhibited increased growth on purines and reduced growth on N-acetyl-D-glucosamine and D-mannose, when used as a sole carbon source. Both mutants grew more poorly on pyrimidines and L-histidine as sole nitrogen source. Several intermediates of the tricarboxylic acid and the urea cycle, as well as several dipeptides, provided differential growth conditions for the two mutants. Gene expression was determined for selected genes and correlated with the observed phenotypes. Phenotypes relating to virulence were determined with the chicken embryo lethality assay. The assay that was previously established for Escherichia coli strains was modified for Y. enterocolitica. The flhD mutant caused reduced chicken embryo lethality when compared to wild-type bacteria. In contrast, the fliA mutant caused wild-type lethality. This indicates that the virulence phenotype of the flhD mutant might be due to genes that are regulated by FlhD/FlhC but not FliA, such as those that encode the flagellar type III secretion system.
conclusionPhenotypes of flhD and fliA mutants are related to central metabolism and virulence and correlate with gene regulation.
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