ArticleIn vitro cellular & developmental biology. Animal
A novel immortalization vector for the establishment of penaeid shrimp cell lines.
Article in In vitro cellular & developmental biology. Animal. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 33 citations in OpenAlex.
- Challenges in cellular agriculture: lessons from Pacific white shrimp, Litopenaeus vannamei.In vitro cellular & developmental biology. Animal · 2025Article
- Establishment of hematopoietic tissue primary cell cultures from the giant freshwater prawnCytotechnology · 2021Article
- Transgene expression in Penaeus monodon cells: evaluation of recombinant baculoviral vectors with shrimp specific hybrid promoters.Cytotechnology · 2016Article
- Establishment of shrimp cell lines: perception and orientation.Indian journal of virology : an official organ of Indian Virological Society · 2012Article
- Pantropic retroviruses as a transduction tool for sea urchin embryos.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Cell cultures from marine invertebrates: new insights for capturing endless stemness.Marine biotechnology (New York, N.Y.) · 2011Review
- Transduction of Schistosoma japonicum schistosomules with vesicular stomatitis virus glycoprotein pseudotyped murine leukemia retrovirus and expression of reporter human telomerase reverse transcriptase in the transgenic schistosomes.Molecular and biochemical parasitology · 2010Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
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Abstract
Cell immortalization technology based on gene transfer has been successfully used to generate cell lines from a wide variety of cell types. The inability to stably introduce and express foreign genes has hampered application of this strategy in shrimp cells. We report here the use of replication-defective pantropic retrovirus to achieve a novel immortalization vector in which simian virus 40 large T antigen (SV40T) gene is expressed from Moloney murine leukemia virus (MoMLV) promoter. Data confirmed the presence of transferred SV40T gene and its stable mRNA expression in transduced lymphoid cells of Penaeus chinensis. The transduced cells showed a higher growth rate and a longer replication life-span compared with their untransduced counterparts. These results indicate the pantropic retrovirus-based immortalization-inducing gene delivery system is a potential tool for establishing cell lines from shrimp.
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