ArticlePloS one2008
Identification of novel pro-migratory, cancer-associated genes using quantitative, microscopy-based screening.
Article in PloS one, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 22 citations in OpenAlex.
- Transcriptional Heterogeneity Underlying Cancer Cell Migration Converges on Shared Regulatory Programs.Genes · 2026Article
- Systematic analysis of migration factors by MigExpress identifies essential cell migration control genes in non-small cell lung cancer.Molecular oncology · 2021Article
- Tracking Single Cells Motility on Different Substrates.Methods and protocols · 2020Article
- Uncovering the signaling landscape controlling breast cancer cell migration identifies novel metastasis driver genes.Nature communications · 2019Article
- A generic methodological framework for studying single cell motility in high-throughput time-lapse data.Bioinformatics (Oxford, England) · 2015Article
- Tumor cell migration screen identifies SRPK1 as breast cancer metastasis determinant.The Journal of clinical investigation · 2015Article
- Mcl-1 promotes lung cancer cell migration by directly interacting with VDAC to increase mitochondrial Ca2+ uptake and reactive oxygen species generation.Cell death & disease · 2014Article
- Fascin is involved in the chemotherapeutic resistance of breast cancer cells predominantly via the PI3K/Akt pathway.British journal of cancer · 2014Article
- Polymeric Aqueous Biphasic System Rehydration Facilitates High Throughput Cell Exclusion Patterning For Cell Migration Studies.Advanced functional materials · 2011Article
- Fascin is a key regulator of breast cancer invasion that acts via the modification of metastasis-associated molecules.PloS one · 2011Article
- Systems microscopy approaches to understand cancer cell migration and metastasis.Cellular and molecular life sciences : CMLS · 2010Review
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundCell migration is a highly complex process, regulated by multiple genes, signaling pathways and external stimuli. To discover genes or pharmacological agents that can modulate the migratory activity of cells, screening strategies that enable the monitoring of diverse migratory parameters in a large number of samples are necessary. METHODOLOGY: In the present study, we describe the development of a quantitative, high-throughput cell migration assay, based on a modified phagokinetic tracks (PKT) procedure, and apply it for identifying novel pro-migratory genes in a cancer-related gene library. In brief, cells are seeded on fibronectin-coated 96-well plates, covered with a monolayer of carboxylated latex beads. Motile cells clear the beads, located along their migratory paths, forming tracks that are visualized using an automated, transmitted-light screening microscope. The tracks are then segmented and characterized by multi-parametric, morphometric analysis, resolving a variety of morphological and kinetic features.
conclusionsIn this screen we identified 4 novel genes derived from breast carcinoma related cDNA library, whose over-expression induces major alteration in the migration of the stationary MCF7 cells. This approach can serve for high throughput screening for novel ways to modulate cellular migration in pathological states such as tumor metastasis and invasion.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.