ArticleMolecular and cellular biology2008
Barhl1 regulatory sequences required for cell-specific gene expression and autoregulation in the inner ear and central nervous system.
Article in Molecular and cellular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 42 citations in OpenAlex.
- Article
- Review
- Hair cell-specific Myo15 promoter-mediated gene therapy rescues hearing in DFNB9 mouse model.Molecular therapy. Nucleic acids · 2024Article
- The evolution of the various structures required for hearing inIBRO neuroscience reports · 2023Article
- Molecular mechanisms governing development of the hindbrain choroid plexus and auditory projection: A validation of the seminal observations of Wilhelm His.IBRO neuroscience reports · 2022Review
- An Integrated Perspective of Evolution and Development: From Genes to Function to Ear, Lateral Line and Electroreception.Diversity · 2021Article
- T-Cell Factors as Transcriptional Inhibitors: Activities and Regulations in Vertebrate Head Development.Frontiers in cell and developmental biology · 2021Review
- A Critical E-box inCells · 2019Article
- Gene, cell, and organ multiplication drives inner ear evolution.Developmental biology · 2017Review
- Article
- Article
- BarTeL, a Genetically Versatile, Bioluminescent and Granule Neuron Precursor-Targeted Mouse Model for Medulloblastoma.PloS one · 2016Article
- The Role of Atonal Factors in Mechanosensory Cell Specification and Function.Molecular neurobiology · 2015Article
- Neurog1 can partially substitute for Atoh1 function in hair cell differentiation and maintenance during organ of Corti development.Development (Cambridge, England) · 2015Article
- Inner ear development: building a spiral ganglion and an organ of Corti out of unspecified ectoderm.Cell and tissue research · 2015Review
- Nonviral Reprogramming of Human Wharton's Jelly Cells Reveals Differences Between ATOH1 Homologues.Tissue engineering. Part A · 2015Article
- SHIELD: an integrative gene expression database for inner ear research.Database : the journal of biological databases and curation · 2015Article
- Article
- Atoh1 directs hair cell differentiation and survival in the late embryonic mouse inner ear.Developmental biology · 2013Article
- Correct timing of proliferation and differentiation is necessary for normal inner ear development and auditory hair cell viability.Developmental dynamics : an official publication of the American Association of Anatomists · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The development of the nervous system requires the concerted actions of multiple transcription factors, yet the molecular events leading to their expression remain poorly understood. Barhl1, a mammalian homeodomain transcription factor of the BarH class, is expressed by developing inner ear hair cells, cerebellar granule cells, precerebellar neurons, and collicular neurons. Targeted gene inactivation has demonstrated a crucial role for Barhl1 in the survival and/or migration of these sensory cells and neurons. Here we report the regulatory sequences of Barhl1 necessary for directing its proper spatiotemporal expression pattern in the inner ear and central nervous system (CNS). Using a transgenic approach, we have found that high-level and cell-specific expression of Barhl1 within the inner ear and CNS depends on both its 5' promoter and 3' enhancer sequences. Further transcriptional, binding, and mutational analyses of the 5' promoter have identified two homeoprotein binding motifs that can be occupied and activated by Barhl1. Moreover, proper Barhl1 expression in inner ear hair cells and cerebellar and precerebellar neurons requires the presence of Atoh1. Together, these data delineate useful Barhl1 regulatory sequences that direct strong and specific gene expression to inner ear hair cells and CNS sensory neurons, establish a role for autoregulation in the maintenance of Barhl1 expression, and identify Atoh1 as a key upstream regulator.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.