ArticleMolecular and cellular biology2008
Chp1-Tas3 interaction is required to recruit RITS to fission yeast centromeres and for maintenance of centromeric heterochromatin.
Article in Molecular and cellular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 48 citations in OpenAlex.
- RNA quality control factors nucleate Clr4/SUV39H and trigger constitutive heterochromatin assembly.Cell · 2024Article
- Subtelomeric Chromatin in the Fission YeastMicroorganisms · 2021Review
- Asterix/Gtsf1 links tRNAs and piRNA silencing of retrotransposons.Cell reports · 2021Article
- Surprising phenotypic diversity of cancer-associated mutations of Gly 34 in the histone H3 tail.eLife · 2021Article
- Native Chromatin Proteomics Reveals a Role for Specific Nucleoporins in Heterochromatin Organization and Maintenance.Molecular cell · 2020Article
- Regulation of centromeric heterochromatin in the cell cycle by phosphorylation of histone H3 tyrosine 41.Current genetics · 2019Review
- Regulation of transcriptional silencing and chromodomain protein localization at centromeric heterochromatin by histone H3 tyrosine 41 phosphorylation in fission yeast.Nucleic acids research · 2018Article
- Article
- Ablation of RNA interference and retrotransposons accompany acquisition and evolution of transposases to heterochromatin protein CENPB.Molecular biology of the cell · 2017Article
- Review
- Nucleation and spreading of a heterochromatic domain in fission yeast.Nature communications · 2016Article
- The Chp1 chromodomain binds the H3K9me tail and the nucleosome core to assemble heterochromatin.Cell discovery · 2016Article
- Control of Chromatin Structure by Long Noncoding RNA.Trends in cell biology · 2015Review
- Chromosome segregation and organization are targets of 5'-Fluorouracil in eukaryotic cells.Cell cycle (Georgetown, Tex.) · 2015Article
- Regulation of histone methylation by noncoding RNAs.Biochimica et biophysica acta · 2014Review
- The RFTS domain of Raf2 is required for Cul4 interaction and heterochromatin integrity in fission yeast.PloS one · 2014Article
- The CINs of the centromere.Biochemical Society transactions · 2013Review
- Sir2 is required for Clr4 to initiate centromeric heterochromatin assembly in fission yeast.The EMBO journal · 2013Article
- Mutations disrupting histone methylation have different effects on replication timing in S. pombe centromere.PloS one · 2013Article
- Clinical impact of proximal autosomal imbalances.Balkan journal of medical genetics : BJMG · 2012Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The maintenance of centromeric heterochromatin in fission yeast relies on the RNA interference-dependent complexes RITS (RNA-induced transcriptional silencing complex) and RDRC (RNA-directed RNA polymerase complex), which cooperate in a positive feedback loop to recruit high levels of histone H3 K9 methyltransferase activity to centromeres and to promote the assembly and maintenance of centromeric heterochromatin. However, it is unclear how these complexes are targeted to chromatin. RITS comprises Chp1, which binds K9-methylated histone H3; Ago1, which binds short interfering (siRNAs); the adaptor protein Tas3, which links Ago1 to Chp1; and centromeric siRNAs. We have generated mutants in RITS to determine the contribution of the two potential chromatin-targeting proteins Chp1 and Ago1 to the centromeric recruitment of RITS. Mutations in Tas3 that disrupt Ago1 binding are permissive for RITS recruitment and maintain centromeric heterochromatin, but the role of Tas3's interaction with Chp1 is unknown. Here, we define the Chp1 interaction domain of Tas3. A strain expressing a tas3 mutant that cannot bind Chp1 (Tas3(Delta)(10-24)) failed to maintain centromeric heterochromatin, with a loss of centromeric siRNAs, a failure to recruit RITS and RDRC to centromeres, and high levels of chromosome loss. These findings suggest a pivotal role for Chp1 and its association with Tas3 for the recruitment of RITS, RDRC, and histone H3 K9 methyltransferase activity to centromeres.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.