ArticleMicrobial cell factories2008
Coutilization of glucose and glycerol enhances the production of aromatic compounds in an Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system.
Article in Microbial cell factories, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 203 citations in OpenAlex.
- Transcriptional and Metabolic Response of a Strain ofBiotech (Basel (Switzerland)) · 2024Article
- Glucose Transport inMicroorganisms · 2023Review
- Rewiring the microbial metabolic network for efficient utilization of mixed carbon sources.Journal of industrial microbiology & biotechnology · 2021Article
- Development and optimization of a microbial co-culture system for heterologous indigo biosynthesis.Microbial cell factories · 2021Article
- Bioprocess Optimization for the Production of Aromatic Compounds With Metabolically Engineered Hosts: Recent Developments and Future Challenges.Frontiers in bioengineering and biotechnology · 2020Review
- Combinatorial expression of different β-carotene hydroxylases and ketolases in Escherichia coli for increased astaxanthin production.Journal of industrial microbiology & biotechnology · 2019Article
- Metabolic engineering for improving L-tryptophan production in Escherichia coli.Journal of industrial microbiology & biotechnology · 2019Review
- Metabolic modeling and response surface analysis of an Escherichia coli strain engineered for shikimic acid production.BMC systems biology · 2018Article
- A Novel Process for Cadaverine Bio-Production Using a Consortium of Two EngineeredFrontiers in microbiology · 2018Article
- Metabolic Engineering Strategies for Co-Utilization of Carbon Sources in Microbes.Bioengineering (Basel, Switzerland) · 2016Review
- Elucidation of the co-metabolism of glycerol and glucose in Escherichia coli by genetic engineering, transcription profiling, and (13)C metabolic flux analysis.Biotechnology for biofuels · 2016Article
- Recombinant expression of glpK and glpD genes improves the accumulation of shikimic acid in E. coli grown on glycerol.World journal of microbiology & biotechnology · 2014Article
- Engineering Escherichia coli to overproduce aromatic amino acids and derived compounds.Microbial cell factories · 2014Review
- Improvement of L-phenylalanine production from glycerol by recombinant Escherichia coli strains: the role of extra copies of glpK, glpX, and tktA genes.Microbial cell factories · 2014Article
- Physiological and transcriptional characterization of Escherichia coli strains lacking interconversion of phosphoenolpyruvate and pyruvate when glucose and acetate are coutilized.Biotechnology and bioengineering · 2014Article
- Constitutive expression of selected genes from the pentose phosphate and aromatic pathways increases the shikimic acid yield in high-glucose batch cultures of an Escherichia coli strain lacking PTS and pykF.Microbial cell factories · 2013Article
- Combining genotype improvement and statistical media optimization for isoprenoid production in E. coli.PloS one · 2013Article
- Article
- New insights into Escherichia coli metabolism: carbon scavenging, acetate metabolism and carbon recycling responses during growth on glycerol.Microbial cell factories · 2012Article
- Improved p-hydroxybenzoate production by engineered Pseudomonas putida S12 by using a mixed-substrate feeding strategy.Applied microbiology and biotechnology · 2011Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEscherichia coli strains lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system (PTS) are capable of coutilizing glucose and other carbon sources due to the absence of catabolite repression by glucose. In these strains, the lack of this important regulatory and transport system allows the coexistence of glycolytic and gluconeogenic pathways. Strains lacking PTS have been constructed with the goal of canalizing part of the phosphoenolpyruvate (PEP) not consumed in glucose transport to the aromatic pathway. The deletion of the ptsHIcrr operon inactivates PTS causing poor growth on this sugar; nonetheless, fast growing mutants on glucose have been isolated (PB12 strain). However, there are no reported studies concerning the growth potential of a PTS- strain in mixtures of different carbon sources to enhance the production of aromatics compounds.
resultsPB12 strain is capable of coutilizing mixtures of glucose-arabinose, glucose-gluconate and glucose-glycerol. This capacity increases its specific growth rate (mu) given that this strain metabolizes more moles of carbon source per unit time. The presence of plasmids pRW300aroGfbr and pCLtktA reduces the mu of strain PB12 in all mixtures of carbon sources, but enhances the productivity and yield of aromatic compounds, especially in the glucose-glycerol mixture, as compared to glucose or glycerol cultures. No acetate was detected in the glycerol and the glucose-glycerol batch fermentations.
conclusionDue to the lack of catabolite repression, PB12 strain carrying multicopy plasmids containing tktA and aroGfbr genes is capable of coutilizing glucose and other carbon sources; this capacity, reduces its mu but increases the production of aromatic compounds.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.