ArticleNucleic acids research2008
Short-hairpin RNAs synthesized by T7 phage polymerase do not induce interferon.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- Thermostability, Tunability, and Tenacity of RNA as Rubbery Anionic Polymeric Materials in Nanotechnology and Nanomedicine-Specific Cancer Targeting with Undetectable Toxicity.Chemical reviews · 2021Review
- Genetically modified pigs are protected from classical swine fever virus.PLoS pathogens · 2018Article
- A Simple and Cost-Effective Approach for In Vitro Production of Sliced siRNAs as Potent Triggers for RNAi.Molecular therapy. Nucleic acids · 2017Article
- Sphingolipids are required for efficient triacylglycerol loss in conjugated linoleic Acid treated adipocytes.PloS one · 2015Article
- Mouse embryonic stem cells have underdeveloped antiviral mechanisms that can be exploited for the development of mRNA-mediated gene expression strategy.Stem cells and development · 2014Article
- Master sensors of pathogenic RNA - RIG-I like receptors.Immunobiology · 2013Review
- Reduced DICER1 elicits an interferon response in endometrial cancer cells.Molecular cancer research : MCR · 2012Article
- Cross regulation of sirtuin 1, AMPK, and PPARγ in conjugated linoleic acid treated adipocytes.PloS one · 2012Article
- Immune signaling by RIG-I-like receptors.Immunity · 2011Review
- Selection of potent non-toxic inhibitory sequences from a randomized HIV-1 specific lentiviral short hairpin RNA library.PloS one · 2010Article
- The chase for the RIG-I ligand--recent advances.Molecular therapy : the journal of the American Society of Gene Therapy · 2010Review
- Sequence-non-specific effects of RNA interference triggers and microRNA regulators.Nucleic acids research · 2010Review
- RNA interference targeted to the conserved dimerization initiation site (DIS) of HIV-1 restricts virus escape mutation.Journal of biochemistry · 2009Article
- Article
- MicroRNAs: key modulators of posttranscriptional gene expression.Gastroenterology · 2009Review
- RNA interference for CFTR attenuates lung fluid absorption at birth in rats.Respiratory research · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA interference (RNAi) mediated by small-interfering RNAs (siRNAs) is a highly effective gene-silencing mechanism with great potential for gene-therapeutic applications. siRNA agents also exert non-target-related biological effects and toxicities, including immune-system stimulation. Specifically, siRNA synthesized from the T7 RNA polymerase system triggers a potent induction of type-I interferon (IFN) in a variety of cells. Single-stranded RNA also stimulates innate cytokine responses in mammals. We found that pppGn (n = 2,3) associated with the 5'-end of the short-hairpin RNA (shRNA) from the T7 RNA polymerase system did not induce detectable amounts of IFN. The residual amount of guanine associated with the 5'-end and hairpin structures of the transcript was proportional to the reduction of the IFN response. Here we describe a T7 pppGn (n = 2,3) shRNA synthesis that does not induce the IFN response, and maintains the full efficacy of siRNA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.