ArticleBMC genomics2008
DArT markers: diversity analyses and mapping in Sorghum bicolor.
Article in BMC genomics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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60 citing papers in PubMed.
- Characterization of spelt wheat (Triticum spelta L.) genotypes using DArTseq technology.Journal of applied genetics · 2026Article
- Dissecting fruit weight and quality traits in Australian passion fruit through genetic linkage mapping and QTL analysis.Frontiers in plant science · 2026Article
- Exploring Genetic Diversity and Population Structure of Australian Passion Fruit Germplasm.Biotech (Basel (Switzerland)) · 2025Article
- Genetic diversity and population structure of soybean (Glycine max (L.) Merril) germplasm.PloS one · 2025Article
- Genomic resources, opportunities, and prospects for accelerated improvement of millets.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Review
- Article
- Recent advancements in the breeding of sorghum crop: current status and future strategies for marker-assisted breeding.Frontiers in genetics · 2023Review
- Genetic and genomic diversity in the sorghum gene bank collection of Uganda.BMC plant biology · 2022Article
- Genetic Diversity and Population Structure of Myanmar Rice (Plants (Basel, Switzerland) · 2021Article
- Article
- Article
- QTL mapping and validation of fertility restoration in West African sorghum ATAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2018Article
- Article
- Ultra-high-throughput DArTseq-based silicoDArT and SNP markers for genomic studies in macadamia.PloS one · 2018Article
- Sweet sorghum as biofuel feedstock: recent advances and available resources.Biotechnology for biofuels · 2017Review
- Root trait diversity, molecular marker diversity, and trait-marker associations in a core collection of Lupinus angustifolius.Journal of experimental botany · 2016Article
- Molecular Approaches to Understand Nutritional Potential of Coarse Cereals.Current genomics · 2016Article
- Mapping QTLs and association of differentially expressed gene transcripts for multiple agronomic traits under different nitrogen levels in sorghum.BMC plant biology · 2016Article
- Article
- DArT Markers Effectively Target Gene Space in the Rye Genome.Frontiers in plant science · 2016Article
Corrections and comments
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Authors and funding
8 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundThe sequential nature of gel-based marker systems entails low throughput and high costs per assay. Commonly used marker systems such as SSR and SNP are also dependent on sequence information. These limitations result in high cost per data point and significantly limit the capacity of breeding programs to obtain sufficient return on investment to justify the routine use of marker-assisted breeding for many traits and particularly quantitative traits. Diversity Arrays Technology (DArT) is a cost effective hybridisation-based marker technology that offers a high multiplexing level while being independent of sequence information. This technology offers sorghum breeding programs an alternative approach to whole-genome profiling. We report on the development, application, mapping and utility of DArT markers for sorghum germplasm.
resultsA genotyping array was developed representing approximately 12,000 genomic clones using PstI+BanII complexity with a subset of clones obtained through the suppression subtractive hybridisation (SSH) method. The genotyping array was used to analyse a diverse set of sorghum genotypes and screening a Recombinant Inbred Lines (RIL) mapping population. Over 500 markers detected variation among 90 accessions used in a diversity analysis. Cluster analysis discriminated well between all 90 genotypes. To confirm that the sorghum DArT markers behave in a Mendelian manner, we constructed a genetic linkage map for a cross between R931945-2-2 and IS 8525 integrating DArT and other marker types. In total, 596 markers could be placed on the integrated linkage map, which spanned 1431.6 cM. The genetic linkage map had an average marker density of 1/2.39 cM, with an average DArT marker density of 1/3.9 cM.
conclusionWe have successfully developed DArT markers for Sorghum bicolor and have demonstrated that DArT provides high quality markers that can be used for diversity analyses and to construct medium-density genetic linkage maps. The high number of DArT markers generated in a single assay not only provides a precise estimate of genetic relationships among genotypes, but also their even distribution over the genome offers real advantages for a range of molecular breeding and genomics applications.
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