ArticleTraffic (Copenhagen, Denmark)2008
Yeast Arf3p modulates plasma membrane PtdIns(4,5)P2 levels to facilitate endocytosis.
Article in Traffic (Copenhagen, Denmark), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 35 citations in OpenAlex.
- The conserved protein adaptors CALM/AP180 and FCHo1/2 cooperatively recruit Eps15 to promote the initiation of clathrin-mediated endocytosis in yeast.PLoS biology · 2024Article
- The GTPase Arf1 Is a Determinant of Yeast Vps13 Localization to the Golgi Apparatus.International journal of molecular sciences · 2021Article
- The protein architecture of the endocytic coat analyzed by FRET microscopy.Molecular systems biology · 2020Article
- Phosphatidylinositol 4,5-bisphosphate in the Control of Membrane Trafficking.International journal of biological sciences · 2020Review
- Yeast-model-based study identified myosin- and calcium-dependent calmodulin signalling as a potential target for drug intervention in chorea-acanthocytosis.Disease models & mechanisms · 2019Article
- Control of morphology and virulence by ADP-ribosylation factors (Arf) in Mucor circinelloides.Current genetics · 2018Article
- Conditional Expression of the Small GTPase ArfA Impacts Secretion, Morphology, Growth, and Actin Ring Position inFrontiers in microbiology · 2018Article
- Role of Arf GTPases in fungal morphogenesis and virulence.PLoS pathogens · 2017Article
- Snf1/AMP-activated protein kinase activates Arf3p to promote invasive yeast growth via a non-canonical GEF domain.Nature communications · 2015Article
- Time resolved DNA occupancy dynamics during the respiratory oscillation uncover a global reset point in the yeast growth program.Microbial cell (Graz, Austria) · 2014Article
- An Abp1-dependent route of endocytosis functions when the classical endocytic pathway in yeast is inhibited.PloS one · 2014Article
- Arf3p GTPase is a key regulator of Bud2p activation for invasive growth in Saccharomyces cerevisiae.Molecular biology of the cell · 2013Article
- The dual PH domain protein Opy1 functions as a sensor and modulator of PtdIns(4,5)P₂ synthesis.The EMBO journal · 2012Article
- Clathrin-mediated endocytosis in budding yeast.Trends in cell biology · 2012Review
- Fission yeast ucp3 gene encodes a putative Arf6 GTPase-activating protein.Molecular biology reports · 2011Article
- ARF family G proteins and their regulators: roles in membrane transport, development and disease.Nature reviews. Molecular cell biology · 2011Review
- ADP-ribosylation factor machinery mediates endocytosis in plant cells.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Endocytic machinery protein SlaB is dispensable for polarity establishment but necessary for polarity maintenance in hyphal tip cells of Aspergillus nidulans.Eukaryotic cell · 2010Article
- Article
- Functions of actin in endocytosis.Cellular and molecular life sciences : CMLS · 2009Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Phosphatidylinositol-(4,5)-bisphosphate [PtdIns(4,5)P2] is a key regulator of endocytosis. PtdIns(4,5)P2 generation at the plasma membrane in yeast is mediated by the kinase Mss4p, but the mechanism underlying the temporal and spatial activation of Mss4p to increase formation of PtdIns(4,5)P2 at appropriate sites is not known. Here, we show that ADP ribosylation factor (Arf)3p, the yeast homologue of mammalian Arf6, is necessary for wild-type levels of PtdIns(4,5)P2 at the plasma membrane. Arf3p localizes to dynamic spots at the membrane, and the behaviour of these is consistent with it functioning in concert with endocytic machinery. Localization of Arf3p is disrupted by deletion of genes encoding an ArfGAP homology protein Gts1p and a guanine nucleotide exchange factor Yel1p. Significantly, deletion of arf3 causes a reduction in PtdIns(4,5)P2 at the plasma membrane, while increased levels of active Arf3p, caused by deletion of the GTPase-activating protein Gts1, increase PtdIns(4,5)P2 levels. Furthermore, elevated Arf3p correlates with an increase in the number of endocytic sites. Our data provide evidence for a mechanism in yeast to positively regulate plasma membrane production of PtdIns(4,5)P2 levels and that these changes impact on endocytosis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.