ArticleHelicobacter2008
Evaluation of nested PCR in detection of Helicobacter pylori targeting a highly conserved gene: HSP60.
Article in Helicobacter, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT06273384 (Diagnostic Performance of Current Infection Marker), which is not on this map. Cited by 25 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Diagnostic Performance of Current Infection Marker (CIM) for Helicobacter Pylori Infection in Patients With Peptic Ulcer Bleeding
Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 56 citations in OpenAlex.
- Pooled it
- Myco-synthesized silver nanoparticles from Aspergillus niger AH1 as multifunctional anti-Helicobacter pylori agents: Antibacterial, antibiofilm, anti-urease, and antibiotic-synergistic activities.Folia microbiologica · 2026Article
- The link betweenAnnals of hepato-biliary-pancreatic surgery · 2023Review
- Development of diagnostic tests for pathogen identification and detection of antimicrobial resistance on WHO global priority pathogens using modular real-time nucleic acid amplification test.International microbiology : the official journal of the Spanish Society for Microbiology · 2023Article
- Outer Membrane Vesicles (OMVs) as Biomedical Tools and Their Relevance as Immune-Modulating Agents againstInternational journal of molecular sciences · 2023Review
- Helicobacter pylori Infection, Its Laboratory Diagnosis, and Antimicrobial Resistance: a Perspective of Clinical Relevance.Clinical microbiology reviews · 2022Review
- Comparison of invasive histological and molecular methods in the diagnosis ofF1000Research · 2022Article
- Phenotype and Molecular Detection of Clarithromycin and Levofloxacin Resistance inInfection and drug resistance · 2020Article
- Upregulation of theGenes & diseases · 2019Article
- MixedHealthcare (Basel, Switzerland) · 2019Article
- Up-regulation of tumor necrosis factor-α pathway survival genes and of the receptor TNFR2 in gastric cancer.World journal of gastrointestinal oncology · 2019Article
- Is There a Correlation betweenMiddle East journal of digestive diseases · 2018Article
- Low Rate ofJournal of clinical and diagnostic research : JCDR · 2017Article
- Helicobacter Pylori Infection: Diagnostic Strategies in Primary Diagnosis and After Therapy.Digestive diseases and sciences · 2016Review
- Prevalence of Helicobacter pylori in children in eastern Turkey and molecular typing of isolates.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2015Article
- [Molecular diagnostics of Helicobacter pylori by PCR in patients in gastroenterology consultation at Saint Camille Medical Centre in Ouagadougou].The Pan African medical journal · 2015Article
- Molecular oncology testing in resource-limited settings.The Journal of molecular diagnostics : JMD · 2014Review
- Diagnosis of Helicobacter pylori: what should be the gold standard?World journal of gastroenterology · 2014Review
- Helicobacter pylori is not eradicated after triple therapy: a nested PCR based study.BioMed research international · 2014Article
- Evaluation of the relationship between dietary factors, CagA-positive Helicobacter pylori infection, and RUNX3 promoter hypermethylation in gastric cancer tissue.World journal of gastroenterology · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
Abstract
objectiveTo comparatively evaluate a new nested set of primers designed for the detection of Helicobacter pylori targeting a highly conserved heat shock protein gene (Hsp60).
methodsA total of 60 subjects having peptic ulcer diseases were tested for the detection of H. pylori using rapid urease test (RUT), histology, culture, and polymerase chain reaction (PCR) in their antral biopsy specimens. A newly designed Hsp60 gene-based primer set was evaluated against commonly used PCR primers for detection of H. pylori.
resultsForty-six of the 60 study subjects were found positive for culture isolation and all the 46 culture-positive specimens were also positive with Hsp60 gene PCR. Of the 46 culture-positive specimens, 44 were positive for 16S rRNA gene, ureC gene, RUT, and histology whereas only 29 were positive with ureA gene PCR. Of the 14 culture-negative subjects, 10 were positive with 16S rRNA gene, 4 were positive with ureC (glmM) gene PCR, and 2 were positive with RUT and 1 was positive on histology.
conclusionThis study shows that nested amplification targeting Hsp60 gene is the most sensitive and specific with LR+ and LR- values of proportional, variant and 0, respectively, when compared with the other three PCR methods. Also, HSP60 gene-specific nested protocol was the most appropriate for detection of H. pylori in clinical specimens. This is particularly valuable because it can be used as a noninvasive method for detecting H. pylori infection in young children and also, in follow-up studies with peptic ulcer patients, on samples like feces and saliva.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.