ArticleMarine biotechnology (New York, N.Y.)
Inhibition of pds gene expression via the RNA interference approach in Dunaliella salina (Chlorophyta).
Article in Marine biotechnology (New York, N.Y.). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 61 citations in OpenAlex.
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- RNAi-Based Biocontrol Products: Market Status, Regulatory Aspects, and Risk Assessment.Frontiers in insect science · 2021Article
- The Potential of Algal Biotechnology to Produce Antiviral Compounds and Biopharmaceuticals.Molecules (Basel, Switzerland) · 2020Review
- Manipulation of the microalgal chloroplast by genetic engineering for biotechnological utilization as a green biofactory.World journal of microbiology & biotechnology · 2018Review
- Current status and perspectives of genome editing technology for microalgae.Biotechnology for biofuels · 2017Review
- Challenges and opportunities for hydrogen production from microalgae.Plant biotechnology journal · 2016Review
- Genetic Engineering: A Promising Tool to Engender Physiological, Biochemical, and Molecular Stress Resilience in Green Microalgae.Frontiers in plant science · 2016Review
- Transgene Expression in Microalgae-From Tools to Applications.Frontiers in plant science · 2016Review
- Article
- Production of carotenoids by microalgae: achievements and challenges.Photosynthesis research · 2015Review
- In Metabolic Engineering of Eukaryotic Microalgae: Potential and Challenges Come with Great Diversity.Frontiers in microbiology · 2015Review
- The potential of transgenic green microalgae; a robust photobioreactor to produce recombinant therapeutic proteins.World journal of microbiology & biotechnology · 2014Review
- Isolation and characterization of a lycopene ε-cyclase gene of Chlorella (Chromochloris) zofingiensis. Regulation of the carotenogenic pathway by nitrogen and light.Marine drugs · 2012Article
- RNA-mediated silencing in Algae: biological roles and tools for analysis of gene function.Eukaryotic cell · 2011Review
- Enhancement of carotenoids biosynthesis in Chlamydomonas reinhardtii by nuclear transformation using a phytoene synthase gene isolated from Chlorella zofingiensis.Applied microbiology and biotechnology · 2011Article
- Characterization of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene from the halotolerant alga Dunaliella salina and inhibition of its expression by RNAi.Current microbiology · 2009Article
- Biotechnological production of value-added carotenoids from microalgae: Emerging technology and prospects.BioengineeredReview
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To investigate the potential of double-stranded RNA interferencing with gene expression in Dunaliella salina, a plasmid pBIRNAI-Dsa was constructed to express hairpin RNA (hpRNA) containing sequences homologous to phytoene desaturase gene (pds), a key gene in carotenoid biosynthesis, and transformed into D. salina by electroporation. The relative transcription level of pds in pBIRNAI-Dsa-treated cells to nontreated cells was quantitated and the gene silencing efficiency by RNAi was evaluated via real-time polymerase chain reaction (PCR). The transcriptions of pds of the pBIRNAI-Dsa-treated group changed compared to those of the control group, and the 2(-delta deltaC)(T) was lowest on the 7th day, corresponding to 0.281265-fold of the relative pds control transcript; a relatively strong gene inhibition effect was therefore deduced. The transcript of pds may be modulated in a wide range, and a reduced transcription even to 28% of the normal level may be tolerated for its survival. This study shows that dsRNA-mediated genetic interference can induce sequence-specific inhibition of gene expression and pBIRNAI-Dsa can be used for transient suppression of gene expression in D. salina. The aim of this study was to exploit dsRNA-mediated gene silencing and to provide a foundation for gene function research in D. salina.
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