ArticleBritish journal of pharmacology2008
Methionine and its derivatives increase bladder excitability by inhibiting stretch-dependent K(+) channels.
Article in British journal of pharmacology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 39 citations in OpenAlex.
- Pacemaking in the lymphatic system.The Journal of physiology · 2026Review
- Pharmacological Separation of Mechanosensory Mechanisms in Rat Urinary Bladder Ex Vivo.Pharmacology research & perspectives · 2026Article
- Long-term follow-up of TREK-1 KO mice reveals the development of bladder hypertrophy and impaired bladder smooth muscle contractility with age.American journal of physiology. Renal physiology · 2024Article
- Pathophysiology of Overactive Bladder and Pharmacologic Treatments Including β3-Adrenoceptor Agonists -Basic Research Perspectives.International neurourology journal · 2024Article
- Vascular mechanotransduction.Physiological reviews · 2023Review
- The effect of amino acids on the bladder cycle: a concise review.Amino acids · 2022Review
- Association between overactive bladder and serum nerve growth factor concentrations in women with high-grade uterine prolapse.International urogynecology journal · 2021Article
- Urinary bladder smooth muscle ion channels: expression, function, and regulation in health and disease.American journal of physiology. Renal physiology · 2020Review
- Altered detrusor contractility and voiding patterns in mice lacking the mechanosensitive TREK-1 channel.BMC urology · 2019Article
- Altered expression and modulation of the two-pore-domain (KAmerican journal of physiology. Renal physiology · 2017Article
- Targeting Ion Channels: An Important Therapeutic Implication in Gastrointestinal Dysmotility in Patients With Spinal Cord Injury.Journal of neurogastroenterology and motility · 2015Article
- Response of the human detrusor to stretch is regulated by TREK-1, a two-pore-domain (K2P) mechano-gated potassium channel.The Journal of physiology · 2014Article
- Electrophysiology and pharmacology of tandem domain potassium channel TREK-1 related BDNF synthesis in rat astrocytes.Naunyn-Schmiedeberg's archives of pharmacology · 2014Article
- Article
- Intravesical liposome and antisense treatment for detrusor overactivity and interstitial cystitis/painful bladder syndrome.ISRN pharmacology · 2014Review
- TREK-1 currents in smooth muscle cells from pregnant human myometrium.American journal of physiology. Cell physiology · 2013Article
- Functional expression of KCNQ (Kv7) channels in guinea pig bladder smooth muscle and their contribution to spontaneous activity.British journal of pharmacology · 2013Article
- Ionic conductances regulating the excitability of colonic smooth muscles.Neurogastroenterology and motility · 2012Review
- Role of potassium ion channels in detrusor smooth muscle function and dysfunction.Nature reviews. Urology · 2011Review
- Colitis decreases mechanosensitive K2P channel expression and function in mouse colon sensory neurons.American journal of physiology. Gastrointestinal and liver physiology · 2011Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
background and purposeDuring the bladder filling phase, the volume of the urinary bladder increases dramatically, with only minimal increases in intravesical pressure. To accomplish this, the smooth muscle of the bladder wall must remain relaxed during bladder filling. However, the mechanisms responsible for the stabilization of bladder excitability during stretch are unclear. We hypothesized that stretch-dependent K(+) (TREK) channels in bladder smooth muscle cells may inhibit contraction in response to stretch. EXPERIMENTAL APPROACHES: Bladder tissues from mouse, guinea pig and monkey were used for molecular, patch clamp, mechanical, electrical, Ca(2+) imaging and cystometric responses to methionine and its derivatives, which are putative blockers of stretch-dependent K(+) (SDK) channels. KEY
resultsSDK channels are functionally expressed in bladder myocytes. The single channel conductance of SDK channels is 89pS in symmetrical K(+) conditions and is blocked by L-methionine. Expressed TREK-1 currents are also inhibited by L-methioninol. All three types of bladder smooth muscle cells from mouse, guinea pig and monkey expressed TREK-1 genes. L-methionine, methioninol and methionine methyl ester but not D-methionine increased contractility in concentration-dependent manner. Methioninol further increased contractility and depolarized the membrane in the presence of blockers of Ca(2+)-activated K(+) conductance. L-methionine induced Ca(2+) waves that spread long distances through the tissue under stretched conditions and were associated with strong contractions. In cystometric assays, methioninol injection increased bladder excitability mimicking overactive bladder activity. CONCLUSIONS AND IMPLICATIONS: Methioninol-sensitive K(+) (SDK, TREK-1) channels appear to be important to prevent spread of excitation through the syncitium during bladder filling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.