Evidence map›Paper›PMID 18203853›Full record

ArticleApplied and environmental microbiology2008

Detection of viable but nonculturable Escherichia coli O157:H7 bacteria in drinking water and river water.

Yanming Liu, Ainslie Gilchrist, Jing Zhang, Xing-Fang Li

Abstract readComparative Study
In one paragraph

Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed
6.3field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 130 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
  6. Review
  7. Article
  8. Article
  9. Review
  10. Zooplankton as a Transitional Host forApplied and environmental microbiology · 2022
    Article
  11. Article
  12. VBNC, previously unrecognized in the life cycle ofJournal of oral microbiology · 2022
    Review
  13. Article
  14. Bacterial Disinfection by CuFeNanomaterials (Basel, Switzerland) · 2019
    Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Expression ofFood science and biotechnology · 2018
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Yanming LiuDivision of Analytical and Environmental Toxicology, Department of Laboratory Medicine and Pathology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta T6G 2G3, Canada.
Ainslie Gilchrist
Jing Zhang
Xing-Fang Li
University of Alberta · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A sensitive method for specific detection of viable Escherichia coli O157:H7 cells, including viable but nonculturable (VBNC) cells, in water samples was developed. This method involved capture of the bacterial cells on a low-protein-binding membrane and direct extraction and purification of RNA followed by reverse transcription-PCR and electronic microarray detection of the rfbE and fliC genes of E. coli O157:H7. It detected as few as 1 CFU of E. coli O157:H7 in diluted cultures, 3 to 4 CFU/liter in tap water, 7 CFU/liter in river water, and 50 VBNC cells in 1 liter of river water, demonstrating the best limit of detection reported to date for VBNC cells in environmental water samples.

Indexed as

Water MicrobiologyWater SupplyCarbohydrate EpimerasesEscherichia coli O157Escherichia coli ProteinsFlagellinMicroarray AnalysisReverse Transcriptase Polymerase Chain ReactionRiversTransaminasesCarbohydrate EpimerasesEscherichia coli ProteinsFlagellinFliC protein, E coliperosamine synthetaseTransaminases

Identifiers

PMID18203853
PMCPMC2258616
OpenAlexW2098048871

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.