ArticleJournal of bacteriology2008
Role of the Nfo and ExoA apurinic/apyrimidinic endonucleases in repair of DNA damage during outgrowth of Bacillus subtilis spores.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 37 citations in OpenAlex.
- Germination and Outgrowth ofMicroorganisms · 2025Article
- The Functional Network of PrkC and Its Interaction Proteins inMicroorganisms · 2025Article
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- Multifactorial resistance ofApplied and environmental microbiology · 2024Article
- The Error-Prone Polymerase DnaE2 Mediates the Evolution of Antibiotic Resistance in Persister Mycobacterial Cells.Antimicrobial agents and chemotherapy · 2022Article
- Structural and Functional Characterization of a Unique AP Endonuclease FromFrontiers in microbiology · 2020Article
- Article
- YwqL (EndoV), ExoA and PolA act in a novel alternative excision pathway to repair deaminated DNA bases in Bacillus subtilis.PloS one · 2019Article
- Role of DNA Repair and Protective Components in Bacillus subtilis Spore Resistance to Inactivation by 400-nm-Wavelength Blue Light.Applied and environmental microbiology · 2018Article
- Transcriptional coupling (Mfd) and DNA damage scanning (DisA) coordinate excision repair events for efficient Bacillus subtilis spore outgrowth.MicrobiologyOpen · 2018Article
- The RecA-Dependent SOS Response Is Active and Required for Processing of DNA Damage during Bacillus subtilis Sporulation.PloS one · 2016Article
- The molecular timeline of a reviving bacterial spore.Molecular cell · 2015Article
- Interaction of apurinic/apyrimidinic endonucleases Nfo and ExoA with the DNA integrity scanning protein DisA in the processing of oxidative DNA damage during Bacillus subtilis spore outgrowth.Journal of bacteriology · 2014Article
- Resistance of Bacillus subtilis spore DNA to lethal ionizing radiation damage relies primarily on spore core components and DNA repair, with minor effects of oxygen radical detoxification.Applied and environmental microbiology · 2014Article
- Transcriptomic and phenotypic analysis of paralogous spx gene function in Bacillus anthracis Sterne.MicrobiologyOpen · 2013Article
- Candidate genes that may be responsible for the unusual resistances exhibited by Bacillus pumilus SAFR-032 spores.PloS one · 2013Article
- Roles of endonuclease V, uracil-DNA glycosylase, and mismatch repair in Bacillus subtilis DNA base-deamination-induced mutagenesis.Journal of bacteriology · 2012Article
- Peroxide stimulon and role of PerR in group A Streptococcus.Journal of bacteriology · 2011Article
- Role of the Nfo and ExoA apurinic/apyrimidinic endonucleases in radiation resistance and radiation-induced mutagenesis of Bacillus subtilis spores.Journal of bacteriology · 2011Article
- Mutations in the Bacillus subtilis beta clamp that separate its roles in DNA replication from mismatch repair.Journal of bacteriology · 2010Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Germination and outgrowth are critical steps for returning Bacillus subtilis spores to life. However, oxidative stress due to full hydration of the spore core during germination and activation of metabolism in spore outgrowth may generate oxidative DNA damage that in many species is processed by apurinic/apyrimidinic (AP) endonucleases. B. subtilis spores possess two AP endonucleases, Nfo and ExoA; the outgrowth of spores lacking both of these enzymes was slowed, and the spores had an elevated mutation frequency, suggesting that these enzymes repair DNA lesions induced by oxidative stress during spore germination and outgrowth. Addition of H2O2 also slowed the outgrowth of nfo exoA spores and increased the mutation frequency, and nfo and exoA mutations slowed the outgrowth of spores deficient in either RecA, nucleotide excision repair (NER), or the DNA-protective alpha/beta-type small acid-soluble spore proteins (SASP). These results suggest that alpha/beta-type SASP protect DNA of germinating spores against damage that can be repaired by Nfo and ExoA, which is generated either spontaneously or promoted by addition of H2O2. The contribution of RecA and Nfo/ExoA was similar to but greater than that of NER in repair of DNA damage generated during spore germination and outgrowth. However, nfo and exoA mutations increased the spontaneous mutation frequencies of outgrown spores lacking uvrA or recA to about the same extent, suggesting that DNA lesions generated during spore germination and outgrowth are processed by Nfo/ExoA in combination with NER and/or RecA. These results suggest that Nfo/ExoA, RecA, the NER system, and alpha/beta-type SASP all contribute to the repair of and/or protection against oxidative damage of DNA in germinating and outgrowing spores.
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Registered trials
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