ArticleJournal of bacteriology2008
Functional consequences of amino acid substitutions to GerVB, a component of the Bacillus megaterium spore germinant receptor.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 35 citations in OpenAlex.
- Early activation of bioenergetic metabolism powers bacterial spore germination.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Genomic identification of germinant receptors and phenotypic response to germinants inCurrent research in microbial sciences · 2025Article
- Genetic Suppression Meets Structure Prediction: Probing a Spore Germination Receptor Complex.Journal of bacteriology · 2022Article
- Dormant spores sense amino acids through the B subunits of their germination receptors.Nature communications · 2021Article
- Importance of Individual Germination Receptor Subunits in the Cooperative Function between GerA and Ynd.Journal of bacteriology · 2019Article
- Structural and functional analyses of the N-terminal domain of the A subunit of aProceedings of the National Academy of Sciences of the United States of America · 2019Article
- The orphan germinant receptor protein GerXAO (but not GerX3b) is essential for L-alanine induced germination in Clostridium botulinum Group II.Scientific reports · 2018Article
- Analysis of Germination Capacity and Germinant Receptor (Sub)clusters of Genome-Sequenced Bacillus cereus Environmental Isolates and Model Strains.Applied and environmental microbiology · 2017Article
- Diversity of the Germination Apparatus inFrontiers in microbiology · 2016Article
- Article
- L-alanine-induced germination in Bacillus licheniformis -the impact of native gerA sequences.BMC microbiology · 2014Article
- Investigating the functional hierarchy of Bacillus megaterium PV361 spore germinant receptors.Journal of bacteriology · 2013Article
- Identification of new proteins that modulate the germination of spores of bacillus species.Journal of bacteriology · 2013Article
- Structure-based functional studies of the effects of amino acid substitutions in GerBC, the C subunit of the Bacillus subtilis GerB spore germinant receptor.Journal of bacteriology · 2011Article
- Genome sequences of the biotechnologically important Bacillus megaterium strains QM B1551 and DSM319.Journal of bacteriology · 2011Article
- Effect of amino acid substitutions in the GerAA protein on the function of the alanine-responsive germinant receptor of Bacillus subtilis spores.Journal of bacteriology · 2011Article
- Amino acid residues in the GerAB protein important in the function and assembly of the alanine spore germination receptor of Bacillus subtilis 168.Journal of bacteriology · 2011Article
- Germinant generation from δ-endotoxin of Bacillus thuringiensis strain 1.1.Current microbiology · 2011Article
- Crystal structure of the GerBC component of a Bacillus subtilis spore germinant receptor.Journal of molecular biology · 2010Article
- Identification of a receptor subunit and putative ligand-binding residues involved in the Bacillus megaterium QM B1551 spore germination response to glucose.Journal of bacteriology · 2010Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The extreme metabolic dormancy and resistance properties of spores formed by members of the Bacillus and Clostridium genera are lost upon exposure to a variety of small-molecule germinants. Germinants are known to interact in an as yet undefined manner with cognate receptor complexes that reside in the inner membrane that surrounds the spore protoplast. The receptor itself is a complex of at least three proteins, and in this study we identify amino acid residues, predicted to lie in loop regions of GerVB on the exterior aspect of the membrane, that influence the Bacillus megaterium spore germination response. Three consecutive residues adjacent to putative transmembrane domain 10 (TM10) were demonstrated to mediate to various degrees the proline germinative response while also influencing germination in response to leucine, glucose, and inorganic salts, suggesting that this region may be part of a ligand binding pocket. Alternatively, substitutions in this region may affect the conformation of associated functionally important TM regions. Leucine- and KBr-mediated germination was also influenced by substitutions in other outer loop regions. These observations, when considered with accompanying kinetic analyses that demonstrate cooperativity between germinants, suggest that binding sites for the respective germinants are in close spatial proximity in the receptor but do not overlap. Additionally, proline recognition was conferred to a chimeric receptor when TM regions associated with the putative binding loop were present, indicating that residues in TM9 and/or TM10 of GerVB are also of functional importance in the proline-induced germinative response.
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