ArticleMarine biotechnology (New York, N.Y.)
Oral administration of bacterially expressed VP28dsRNA to protect Penaeus monodon from white spot syndrome virus.
Article in Marine biotechnology (New York, N.Y.). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 137 citations in OpenAlex.
- RNA Interference Applied to Crustacean Aquaculture.Biomolecules · 2024Review
- Experimental Infection Models and Their Usefulness for White Spot Syndrome Virus (WSSV) Research in Shrimp.Viruses · 2024Review
- Shrimp injection with dsRNA targeting the microsporidian EHP polar tube protein reduces internal and external parasite amplification.Scientific reports · 2024Article
- Structural modelling and preventive strategy targeting of WSSV hub proteins to combat viral infection in shrimp Penaeus monodon.PloS one · 2024Article
- Engineering a replication-incompetent viral vector for the delivery of therapeutic RNA in crustaceans.PNAS nexus · 2023Article
- Methods for the Cost-Effective Production of Bacteria-Derived Double-Stranded RNA forFrontiers in physiology · 2022Article
- Potential dsRNAs can be delivered to aquatic for defense pathogens.Frontiers in bioengineering and biotechnology · 2022Review
- Antiviral therapy in shrimp through plant virus VLP containing VP28 dsRNA against WSSV.Beilstein journal of organic chemistry · 2021Article
- Feeding exogenous dsRNA interferes with endogenous sRNA accumulation in Paramecium.DNA research : an international journal for rapid publication of reports on genes and genomes · 2020Article
- RNA Interference by Ingested dsRNA-Expressing Bacteria to Study Shell Biosynthesis and Pigmentation in Crassostrea gigas.Marine biotechnology (New York, N.Y.) · 2019Article
- A new age in AquaMedicine: unconventional approach in studying aquatic diseases.BMC veterinary research · 2018Review
- Trojan Horse Strategy for Non-invasive Interference of Clock Gene in the Oyster Crassostrea gigas.Marine biotechnology (New York, N.Y.) · 2017Article
- VP24 Is a Chitin-Binding Protein Involved in White Spot Syndrome Virus Infection.Journal of virology · 2016Article
- Small Non-coding RNAs Associated with Viral Infectious Diseases of Veterinary Importance: Potential Clinical Applications.Frontiers in veterinary science · 2016Review
- Large-scale production and antiviral efficacy of multi-target double-stranded RNA for the prevention of white spot syndrome virus (WSSV) in shrimp.BMC biotechnology · 2015Article
- Chitosan/interfering RNA nanoparticle mediated gene silencing in disease vector mosquito larvae.Journal of visualized experiments : JoVE · 2015Article
- Inactivated E. coli transformed with plasmids that produce dsRNA against infectious salmon anemia virus hemagglutinin show antiviral activity when added to infected ASK cells.Frontiers in microbiology · 2015Article
- QTL for white spot syndrome virus resistance and the sex-determining locus in the Indian black tiger shrimp (Penaeus monodon).BMC genomics · 2014Article
- Protection of shrimp Penaeus monodon from WSSV infection using antisense constructs.Marine biotechnology (New York, N.Y.) · 2014Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We explored the possibility of protecting Penaeus monodon against white spot syndrome virus (WSSV) infection via interference RNA technology by oral administration of bacterially expressed WSSV VP28dsRNA. Shrimp were given dsRNA orally via two methods. In the first method, pellet feed was coated with inactivated bacteria containing overexpressed dsRNA of the WSSV VP28 gene, and in the second method, pellet feed was coated with VP28dsRNA-chitosan complex nanoparticles. The treated shrimp were orally challenged with WSSV by feeding WSSV-infected tissue. The experiment was conducted for 30 days. The dsRNA-treated shrimp challenged with WSSV showed higher survival compared to control shrimp. Sixty-eight percent survival was observed in shrimp fed with feed coated with inactivated bacteria containing dsRNA of the WSSV VP28 gene whereas 37% survival was observed in shrimp fed with VP28dsRNA-chitosan complex nanoparticle-coated feed. The WSSV caused 100% mortality in shrimp fed with pellet feed coated with inactivated bacteria with empty LITMUS38i vector. At the end of the experiment, the tissue samples prepared from randomly selected shrimp that survived were analyzed via reverse transcriptase-polymerase chain reaction and Western blot analysis for WSSV. The samples were negative for WSSV. Based on the present data and the advantages of dsRNA, we believe that oral administration of crude extract of bacterially expressed VP28dsRNA is a potential therapeutic agent against WSSV infection of shrimp.
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